LIN Wen-chun, YU Shou-wu, RUAN Guan-hai, FAN Ye-yang, XIE Jian-kun
Grain weight is one of the major components of rice yield-associated traits, which is also an important factor effecting grain quality trait. The aim of this study is to finely map a quantitative trait locus (QTL) for thousand-grain weight (TGW). Five residual heterozygous lines (RHLs) carrying heterozygous segments of RM151-RM10404, RM151-RM10425 and RM1344-RM5359 in the almost pure parental background were selected from the recombinant inbred lines of Zhenshan 97/Miyang 46. F2:3 populations of the five RHLs were grown for QTL analysis for TGW,the target region RM151-RM1344 were confirmed existing a QTL for TGW of rice, and the allele for increasing TGW is derived from the parent Zhenshan 97. Using simple sequences repeat marker (SSR) analysis, four individual plants with the heterozygous segments of RM151-RM3746, RM151-RM10402, RM10381-RM10425 and RM10381-RM10425 were selected to develop F2 populations (FF population), and mapped the qTGW1 between RM10376-RM10404. Three individual plants with the heterozygous segments of RM10381-RM10402,RM10390-RM10425 and RM1344-RM10425 were used to develop their F2 populations (L population). Using these four F2 populations for further fine mapping, we narrowed the qtgw1 between RM10381 and RM10404 with a physical distance of about 246.6kb.