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Effects of Nitrogen Application Amount on Formation and Yield of Potato Tuber Starch in Ningxia Arid Region
LIU Qiangjuan, KANG Jianhong, WU Jiarui, SUN Jianbo, MA Xueying, WANG Xingqiang, JIAN Tiancai
2021, 35 (5): 1196-1208. DOI: 10.11869/j.issn.100-8551.2021.05.1196
Abstract151)   HTML8)    PDF (1930KB)(1363)      
For exploring the effect mechanism of different nitrogen application amounts impact on potato tuber starch formation and yield in Ningxia arid areas, field trials were carried out in the mountainous areas of southern Ningxia in 2017(normal year) and 2019(wet year) respectively. The potato cultivar Qingshu No. 9 was used as the material and a single factor random Block experiment was designed, with no nitrogen fertilizer (N0) as the control, to study the effects of pure nitrogen application rate of 75 (N1), 150 (N2), 225 kg·hm -2 (N3) on potato starch content, key starch forming enzymes and yield. The results showed that with the increase of nitrogen application amount, the total starch and amylopectin content increased first and then decreased, with N2 being the highest, significantly increasing by 21.23, 26.42 percentage point (2017) and 19.74、24.93 percentage point(2019); while the amylose content increased with the increase of nitrogen application amount, reaching the highest at N3, which was 1.59 percentage point (2017) and 1.78 percentage point (2019) higher than N0. Both adenosine diphosphate glucose pyrophosphorylase (AGPP) and starch branching enzyme (SBE) had the highest N2, which increased by 57.38%, 36.83% (2017) and 67.19%, 29.01% (2019) compared with N0, respectively; while soluble starch synthase (SSS) and bound starch synthase (GBSS) were the highest with N3, which increased by 33.90%, 49.51% (2017) and 46.02%, 51.00% (2019) respectively compared with N0. Correlation and general analysis show that starch content and key enzymes in starch synthesis have a positive correlation, and AGPP, SSS, and GBSS have a greater direct contribution to the process of starch synthesis; Compared with N0, the potato output of N1~N3 increased by 10.72%~21.02% and 8.40%~16.30% in 2017 and 2019, respectively. The yield of N2 is the best; the best nitrogen application rate to achieve the highest yield is 150-184 (2017), 150-196 kg·hm -2 (2019). Therefore, it is suggested that the nitrogen application amount of 150-200 kg·hm -2 for potato in arid areas of Ningxia can effectively increase the activity of key enzymes in starch formation, accelerate starch formation and accumulation, and promote the increase of potato production, thereby increasing the economic benefits of local people.
Identification of MIR319 Family Members and Their Target Genes in Response to Cucumber Green Mottle Mosaic Virus Infection in Watermelon
SUN Yuyan, ZHANG Huiqing, FAN Min, HE Yanjun, GUO Ping'an
2021, 35 (5): 1048-1059. DOI: 10.11869/j.issn.100-8551.2021.05.1048
Abstract173)   HTML8)    PDF (16143KB)(1338)      
In order to reveal the possible roles of MIR319 family (miR319, miR319a and miR319a-3p) under the CGMMV stress, mature sequences of MIR319 were blasted against the watermelon genome to obtain the precursor gene. MEGA was used to analyze the evolutional relationship of precursor genes for MIR319. PlantCARE was used to analyze the cis-acting regulatory elements of the precursor gene promoter. Degradome sequencing was used to identify target genes of MIR319, and transcriptome sequencing and qRT-PCR were used to obtain the expression of target genes. The common precursor gene of MIR319, Pre-MIR319 was obtained, which was 170 bp in length, able to form the stem-loop structure. Sequence alignment showed that mature sequences of MIR319 were highly conserved at the 2~14 bases of 5'-terminal. Phylogenetic analysis of watermelon Pre-MIR319 and 116 miR319 precursor sequences from 35 species divided these precursor sequences into four branches. Watermelon Pre-MIR319 was closest to potato precursor gene miR319 a (MI0025952). The promoter of Pre-MIR319 contain several cis-acting regulatory elements, such as light responsive element, gibberellin responsive element, ethylene responsive element, methyl jasmonate response element, MYB, MYC and etc. Five target genes of MIR319 family, Cla019567, Cla013523, Cla023342, Cla002428 and Cla013668, were predicted by degradome sequencing results. Among which, Cla019567, Cla013523, Cla023342 and Cla002428 are annotated as TCP transcription factor and Cla013668 is annotated as MYB transcription factor. The cleavage sites were located at the 10 th of MIR319 at the 5'-terminal end. The amino acids number, molecular weight and theoretical isoelectric point of target genes were 319~554 aa, 34.94~61.21 kDa and 5.29~7.80, respectively. These proteins do not contain transmembrane domains and was located in the nucleus/cytoplasm. Expression profiles of target genes using transcriptome and qRT-PCR analysis revealed that miR319a negatively regulated its target gene Cla013523 ( TCP). These results clarified the role of MIR319 family members in CGMMV stress response and revealed the regulation of MIR319 on their target genes.
Transcriptome Analysis and Gene Function Annotation of Early Developmental Broccoli Microspores Based on High-throughput Sequencing Technology
ZHANG Zhenchao,YAO Yuemei,MAO Zhongliang,SUN Guosheng,QIN Wenbin,DAI Zhongliang
2018, 32 (5): 848-855. DOI: 10.11869/j.issn.100-8551.2018.05.0848
Abstract197)   HTML8)    PDF (9287KB)(1149)      
In order to investigate the molecular biological mechanisms of broccoli microspore embryogenesis, the Illumina HiSeq TM 2000 platform was utilized to analyze and annotate transcriptomes of the microspores, cultured at 32.5℃for 17 h and 24 h and at 25℃ for 0, 1, 6 d in this research. The results showed that a total of 174324 Unigenes were produced, among which 144 194 were annotated in GO、COG、KEGG、NR、Swissprot and Pfam database. The analysis of gene ontology function enrichment revealed that some important biological processes related to microspore embryogenesis, such as cell part, cell and organelle, binding and catalytic activity, metabolic process, cellular process and single-organism process, etc, got significantly enriched. The frequencies of R、L、K、T and O were much higher than others in COG function classification. The results of KEGG pathways enrichment showed that differentially expressed genes produced from microspores after heat shock tended to cluster into the pathway of protein processing in endoplasmic reticulum, the pathways of plant-pathogen interaction, plant hormone signal transduction and spiceosome, etc. The results laid a good foundation for further study on the physiological and biochemical and molecular mechanisms of broccoli microspore embryogenesis.
Nitrate Uptake, Transport and Signaling Regulation Pathways
LI Chenyang, KONG Xiangqiang, DONG Hezhong
2020, 34 (5): 982-993. DOI: 10.11869/j.issn.100-8551.2020.05.0982
Abstract333)   HTML14)    PDF (3084KB)(1335)      

Nitrate is the major forms of nitrogen that plants absorb from the soil, and its uptake and utilization is a highly coordinated and complex process. For the sake of survive in various environmental conditions, plants have evolved suitable nitrate uptake and utilization mechanisms to adapt to a wide range of environmental conditions. There are various types of nitrate receptors in plant roots, which can sense different concentrations of external nitrate. The low- or high-affinity nitrate uptake system was activated to absorb nitrate from the environment according to the levels of external nitrate. Once nitrate is taken up into root cells, most of it is transported to shoots for assimilation, and synthesizes macromolecular substances to optimize plant growth. When the nitrate supply is too much for use immediately, the plant can store the excess nitrate into vacuoles and mediate it efflux from vacuoles to the cytosol for assimilation when needed. During plant growth and development, nitrate in the old and mature leaves can be redistributed into developing tissues to promote their growth. Many genes related to nitrate absorption, transportation, storage, assimilation and signaling regulation are activated orderly and work coordinately to absorb and utilize nitrate efficiently. This review summarizes the NRT1 and NRT2 nitrate uptake-related genes and their functions, as well as related transcription factors involved in primary nitrate response and small signal peptides in nitrate signaling transduction and exchange among different tissues. In order to further understand the mechanisms of plant uptake and utilize nitrate, and therefore provides new ideas in increasing nitrogen-use efficiency of crop by breeding and cultivation techniques.

Effect of 60Co-γ Irradiation on Capsule of Epidendium secundum
ZHOU Yaqian, YAO Na, WEI Li, LI Lubin, LIU Lei
2017, 31 (9): 1693-1699. DOI: 10.11869/j.issn.100-8551.2017.09.1693
Abstract250)   HTML5)    PDF (1220KB)(955)      
To investigate the effect of the 60Co-γ ray treatment on the Epidendrum secundum capsule, different radiation doses of 60Co-γ ray (0 to 200 Gy) were used in this study, and the effect of radiation on the seeds germination and the seedling growth were investigated. The results showed that 20 Gy radiation dose can promote the germination rate compared with the control group, and the germination time was shortened. Under the radiation dose of 200Gy, the seeds could not germinate. The semi-lethal dose (LD 50) was 78.08 Gy. The biological statistics analysis indicated the radiation dose had larger impact on plant height and leaf length, and had little effect on blade quantity and less influence on the leaf width. These results will lay the theory basis for radiation breeding of Epidendium secundum.
Evaluation of Soil Nutrient Content and Its Distribution of Cultivated Land in South of Xinjiang: Taking Aksu Prefecture as an Example
TIAN Liwen, QI Yongchun, DAI Lu, CUI Jianping, GUO Rensong, XU Haijiang, LIN Tao, ZHANG Na
2020, 34 (1): 214-223. DOI: 10.11869/j.issn.100-8551.2020.01.0214
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To explore the soil nutrient content and distribution characteristics of cultivated land in south Xinjiang after years of cultivating since the second national land survey in 1982, the Aksu prefecture of xinjiang cotton producing area with typical natural ecological conditions in southern Xinjiang was taken as a representative in our research. Field investigation and laboratory analysis were used to measure the soil major element content of organic matter, total nitrogen, available nitrogen, available phosphorus, available potassium and microelement content of available copper, iron, zinc, manganese, then the determination results were analyzed by combining with relevant indices of soil nutrient grading in Xinjiang. The results showed that compared with 1982, soil nutrient content in the cultivated land was greatly improved. The average organic matter content of the whole region was 13.56 g·kg -1; total nitrogen alkaline hydrolysis nitrogen, available phosphorus and available potassium were 680, 62.30, 13.90 and 145.00 mg·kg -1, respectively. Among them, the organic matter content in Aksu Prefecture was the same as that of in 1982, while the available potassium content was decreased at rate of 2.20 mg·kg -1 annually , and the content of available nitrogen and available phosphorus were higher 74.50% and 385.86% than that of in 1982, respectively. The overall result was as follows: that the content of phosphorus and potassium was abundant, the organic matter was in the middle level, except for the effective manganese content in the lower-middle level; the content of effective copper, available iron and zinc were in different degree of abundant state, and the content of nitrogen was low. In summary, it was recommended to strengthen the implementation of the balanced fertilization program for the current nutrient content levels and trends of soil, and to improve the content of soluble fertilizers such as nitrogen through reasonable irrigation, and prevent soil salinization. Thus, this research provided a theoretical basis for the future agricultural production of soil fertility and scientific fertilization in Aksu prefecture.
Review on Chemical Structures and Biological Activities of Spermidine Derivatives in Plants
ZHAO Shuo, XING Cencan, WANG Yan, SUO Ran, WANG Fengzhong
2018, 32 (1): 123-130. DOI: 10.11869/j.issn.100-8551.2018.01.0123
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Spermidine derivatives, which widely existed in the pollen, leaf and fruit in the plants, are a kind of natural compounds which the hydrogen atom of spermidine is replaced by other functional group. The structural identification, purification, especially the biological activities of spermidine derivatives are still worthy to explore. This review summarizes the chemical structures, sources, purification and biological activities of spermidine derivatives reported in the past 30 years, which may possibly build a foundation for future research of spermidine derivatives with special functions.
Cloning and Expression Analysis of the MYB Transcription Factor RcWER-like in Rosa chinensis
BAO Ying, LI Zeqing, WEI Linyan, CHEN Chao
2020, 34 (6): 1144-1151. DOI: 10.11869/j.issn.100-8551.2020.06.1144
Abstract368)   HTML14)    PDF (8140KB)(959)      
MYB transcription factors play an important regulation role in the salt stress-responsive of plant. In order to characterize the biological function of a MYB transcription factor, RcWER-like, its'expression profile in diverse tissues under salt, hormone, and salt combined with hormore treatment were analyzed by real-time PCR. In this study, a MYB transcription factor gene of rose, RcWER-like was cloned from R. chinensis Old Blush based on the transcriptome and genome data. Bioinformatic analysis showed that the total length of RcWER-like gene was 882 bp, and the ORF of RcWER-like was 669 bp, which encode 223 amino acids. Multi-alignment of RcWER-like with other MYB type proteins revealed that RcWER-like had two conserved domains in the N-terminal. Phylogenic tree analysis showed that RcWER-like was clustered with PpWER-like in Prunus persica, PmWER-like in Prunus mume and MdWER-like in Malus domestica, and belonged to the R2R3-MYB type transcription factor. Real-time PCR results showed that the expression level of RcWER-like was significantly increased at 24 h under salt stress, and the SA and MeJA treatments could also induce the expression of RcWER-like. In addition, under salt stress, RcWER-like was significantly up-regulated by SA and MeJA, which was higher than that under salt stress and exogenous hormone treatment. Meanwhile, there were significant differences in the expression patterns of RcWER-like in different tissues at different time points under the treatment of salt and hormone. These evidences implied that the R2R3-MYB transcription factor RcWER-like in Rosa chinensis Old Blush could response to SA and MeJA, and might play an important role in response to salt stress. The results will provide candidate gene resources and theoretical basis for salt tolerance molecular breeding of rose.
Identification and Analysis of Heat Tolerance Identification and Analysis for Brassica campestris ssp. chinensis
ZHANG Jing-yun, ZHAO Xiao-dong, WAN Xin-jian, XIONG De-tao, HU Xin-long, MIAO Nan-sheng
2014, 28 (1): 146-153. DOI: 10.11869/j.issn.100-8551.2014.01.0146
Abstract359)      PDF (7855KB)(652)      
Sixty-eight varieties of Brassica campestris ssp. chinensis were tested and screened for heat tolerance based on phenotype evaluation in field, and varieties with various heat tolerances were measured for their malondialdehyde content, relative leaf membrane permeability and proline content. The results showed the five tolerant varieties and five sensitive varieties selected were increased in malondialdehyde content, relative leaf membrane permeability and proline content under heat stress as time proceeded, but significant differences were found between the two groups of varieties. Therefore, malondialdehyde content, membrane permeability and proline content could be used as physiological indices for identification of heat tolerance in Brassica campestris ssp. chinensis.
Cloning and Deletion Analysis of NtMTPC4 Promoter From Nicotiana tabacum
LIU Jikai, ZHANG Lin, GAO Yongfeng, WU Chanjuan, TANG Yunlai
2017, 31 (7): 1282-1289. DOI: 10.11869/j.issn.100-8551.2017.07.1282
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Tissue-specific promoters are important tools in genetic engineering and practical application. In order to study the expression characteristics of NtMTPC4 promoter from Nicotiana tabacum, the relative expression of NtMTPC4 was determined by quantitative real-time PCR (qRT-PCR). A 2 287 bp length promoter sequence of NtMTPC4 gene was cloned by PCR method. Based on the distribution of the regulatory elements, three truncations of NtMTPC4-P were obtained. The full length NtMTPC4-P and the truncations were then inserted into pBI121K respectively to replace the CaMV35S promoter. All the recombinant plasmids were introduced into Arabidopsis by Agrobacterium-tumefaciens mediated floral dipping method. qRT-PCR analysis indicated that NtMTPC4 gene was highly expressed in flowers. Sequence analysis showed that NtMTPC4-P contained basic cis-elements, such as CAAT-box, TATA-box and tissue specific regulation elements, GTGANTG10 and POLLEN1LELAT52. GUS histochemical assay showed that the expression of the GUS in all of the transgenic plants was detected particularly in pollens. These results provide a new regulatory element for the expression of target gene specifically in pollen by genetic engineering.
Effects of Different Organic Fertilizer Replacement Rates on Maize Growth and Soil Fertility Under Equal Nutrient Conditions
HE Hao, ZHANG Yutong, WEI Changzhou, LI Junhua
2021, 35 (2): 454-461. DOI: 10.11869/j.issn.100-8551.2021.02.0454
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In order to explore the effect of reducing fertilizer and increasing benefits of maize under different organic fertilizer replacement rates,and determine the optimum replacement rate, field plot experiments were conducted under equal nutrient conditions,and five treatments with no fertilization control(CK),conventional fertilization(CF),and organic fertilizer replacement rates of 8%,16%,and 24%(recorded as 8%OF,16%OF,and 24%OF,respectively)were set up to study the effects of different organic fertilizer replacement rates on maize growth and development,yield,economic benefits and soil fertility.The results showed that compared with CF, the treatments with different organic fertilizer replacement rates promoted the growth and development of maize,and increased the plant height,stem diameter,leaf number and SPAD value of leaves in different growth stages of maize. By effectively improving the ear traits and yield components of maize,the yield increased by 17.56%,28.50% and 9.82%,and the net income than CF was 1130.64,1303.32 and -2774.55 yuan·hm -2,respectively. The physical and chemical properties of soil at maturity stage were improved,the contents of alkaline nitrogen,available phosphorus,available potassium,organic matter and soil water increased by 13.98%~14.89%,7.29%~36.93%, 2.46%~11.20%,11.85%~15.36%, and 10.05%~25.06%,respectively,soil pH and soil bulk density decreased by 0.04~0.17 and 4.61%~7.24%,respectively. Through model analysis,the optimum organic fertilizer replacement rate was determined to be 9.5%,and the fertilization scheme was the application rates of organic fertilizer,chemical fertilizer N,P 2O 5 and K 2O were 1 610.2,271.5,148.6,51.5 kg·hm -2,respectively.This study provides a feasible basis for rational use of nutrient resources and the reduction of fertilizer input in maize production.
Bioinformatics Identification and Analysis of BBX Gene Family in Grape
GE Mengqing, LIU Zhongjie, ZHU Xudong, LU Suwen, GUAN Le, FANG Jinggui
2021, 35 (1): 18-30. DOI: 10.11869/j.issn.100-8551.2021.01.0018
Abstract264)   HTML17)    PDF (9687KB)(679)      
BBX transcription factor family participates in photomorphogenesis, flowering, photoperiod regulation and shade avoidance response of plant seedlings, and plays an important role in the growth and development of higher plants. In this study, bioinformatic analysis of the VvBBX gene family members in grape were conducted, including the number, gene structure, promoter, amino acid characteristics, chromosome location and gene evolution. A total of 25 members of BBX family were identified in grape, which are mainly acidic proteins. Subcellular localization showed that there were four putative secretory pathway signal peptides ( VvBBX2, VvBBX5, VvBBX7 and VvBBX20), two chloroplast transit peptides ( VvBBX23 and VvBBX24), and one mitochondrial targeting peptide ( VvBBX1). Chromosome mapping revealed that the 25 VvBBXs were distributed on 11 chromosomes, including 1, 3, 4, 5, 7, 9, 11, 12, 14, 18 and 19; the results of phylogenetic evolution analysis divided the VvBBX family into five subfamilies; homology analysis revealed that the BBX protein family members were highly conserved between grape and Arabidopsis thaliana; and their promoter regions contained light-related elements and other cis-acting elements response to hormones, low temperatures, and etc. The expression profiles of the 25 VvBBXs in ten grape tissues at different developmental stages showed that the gene family expressed with spatial-temporal specificity. In addition, the relative expression of grape BBX gene family members varied with different light conditions, which indicated that they had a close relationship with photomorphogenesis and photosynthesis. These results provide an important basis for further functional analysis of BBX gene family in grape.
Transcriptome Analysis of the Green Stalk and the White Stalk of Cauliflower
LIN Hui, XUE Zhuzheng, LI Yongping, LI Dazhong, LIU Jianting, ZHU Haisheng, WEN Qingfang
2018, 32 (9): 1708-1720. DOI: 10.11869/j.issn.100-8551.2018.09.1708
Abstract291)   HTML8)    PDF (6081KB)(876)      
In order to explore the genetic basis for the colour of cauliflower curd stalk formation,the green stalk and the white stalk were sequenced by IlluminaHiSeq 4000 platform,a total of 52 253 822 readings were generated.And 66 450 unigenes with 1 285 bp length of N50, were obtained by denovo assembly method, the average unigenes length was 717.40 bp. Annotation analysis of unigene indicated that 45 390 unigenes had homologes in public protein database;However,21 060 sequences had no hits and might be specific in Brassica oleracea L. var. botrytis L. specific. All DEGS were annotated by different databases. By GO database, the 2 540 DEGS were divided into 3 categories containing 54 function groups;by COG databases, 753 DEGS were grouped into 24 functional categories;By KEGG database, 946 DEGS were divided into 119 metabolism pathways,among which 6 DEGS which were involved in Carotenoid biosynthesis pathway,9 DEGS may take part in Flavonoid biosynthesis pathway and 1 DEGS may take part in chlorophyll metabolism pathway and vesponsible for the colour formation. 16 DEGS were verified by qRT?PCR, and showed consistent with the results of RNA?Seq sequencing. These findings provide a scientific basis for further revealing the mechanism of the colour in cauliflower stalk and lay a theoretical foundation for the genetic improvement on cauliflower.
Role of Alternative Splicing in Plant Development and Abiotic Stress Responses
FENG Yalan, XIONG Ying, ZHANG Jun, YUAN Jiale, CAI Aishan, MA Chao
2020, 34 (1): 62-70. DOI: 10.11869/j.issn.100-8551.2020.01.0062
Abstract242)   HTML4)    PDF (1726KB)(982)      
Alternative splicing is one of the molecular mechanisms by which plants regulate their gene interaction networks. It regulates plant development and response to abiotic stresses mainly at the post-transcriptional level, greatly increasing the complexity of the transcriptome and proteome. This review summarizes the molecular mechanisms and action pattern of alternative splicing, as well as its role in plant development and abiotic stress responses. Based on current research status, the future research direction of alternative splicing has being prospected, which may provide theoretical reference for the regulation of plant growth and development as well as the breeding of excellent stress-resistant varieties.
Comparative Study on Sugar Metabolism in Keitt Mango Fruit Between Bagging With Yellow and White Bags During Postharvest
WU Lina, ZHU Yuyan, HUAN Chen, XU Qihang, LI Sheng'e, ZHENG Xiaolin
2021, 35 (5): 1121-1128. DOI: 10.11869/j.issn.100-8551.2021.05.1121
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In order to explore the effects of different colored bagging on postharvest soft nose and storage characteristics, the incidence of soft nose and sugar metabolism in mango fruit Mangifera indica L.cv. Keitt pre-harvest bagged with yellow or white bag were investigated during storage. The results showed that the bagging with yellow bag significantly reduced the incidence of soft nose disease in mango fruit during storage for 10 d compared to bagging with white bags. In addition, the bagging with yellow bags significantly decreased the activities of amylase, acid invertase (AI), neutral invertase (NI), sucrose phosphate synthase (SPS) and sucrose synthase (SS) in flesh of the mango fruit, which resulted in slowing the rates of decrease in starch content and accumulation of soluble sugar content, and in turn to be contributed to delaying the softening rate and improving the fruit storability of mango fruit during storage. It was suggested that the bagging treatment affected the sugar metabolism would be associated with the development of soft nose disease in Keitt mango fruit during postharvest. Our present work might provide references for selecting reasonable pre-harvest bagging materials of mango fruit in practice.
Genome Wide Identification and Expression Analysis of the SBP Gene Family in Foxtail Millet
SONG jian, CAO Xiaoning, WANG Haigang, CHEN ling, WANG Junjie, QIAO Zhijun, LIU Sichen
2020, 34 (7): 1409-1420. DOI: 10.11869/j.issn.100-8551.2020.07.1409
Abstract268)   HTML2)    PDF (3178KB)(731)      
In order to further elucidate the important function of SBP gene family in foxtail millet, NCBI blast and HMMER search were used to identify the SBP genes in foxtail genome, and to analyze the physicochemical properties and phylogenetic tree of SBP family in foxtail millet. The expression SBP gene family in foxtail millet treated with PEG and ABA were detected by real-time fluorescence quantitative PCR (RT-qPCR). The results showed that 19 members of SBP gene family were identified from the whole genome of foxtail millet and they encoded proteins with 199-1 118 aa, which distributed across 9 chromosomes. The results of phylogenetic tree showed that the SBP protein could be divided into 2 categories. The first category was classified into 6 sub-categories, and the second category only contained one SBP proteins. The numbers of exons were variable, ranged from 2-11, and all of the SBP proteins were hydrophilic protein. The results of subcellular localization prediction showed that the members of the gene family were located mainly in the nucleus except SBP7. Cluster analysis showed that foxtail millet SBP was closely related to monocotyledonous sorghum and rice than to cotyledonous Arabidopsis thaliana. Upstream promoter region analysis founded a large number of cis-acting elements closely related to hormones, drough, light, pathogenic factors and stress condition. The results of RT-qPCR showed that the relative expression of SBP gene was up-regulated under PEG and ABA treatment, and the expression quantity of SBP in leaf was higher than that in stem. The expression quantity of SBP under PEG was significantly higher than ABA treatment. The expression levels of SiSBP13 in leaves and stems after 4 hours of PEG stress were 180 and 15 times as that before treatment. After 4 hours of ABA induction, the expression of SiSBP13 in stems was up to 8 times that before stress. After 24 hours of induction, the expression of SiSBP13 in leaves was 28 times higher than that before stress. This study laid an important foundation for further analysis of the specific functions of SBP genes at different development stages in foxtail millet.
Effect of Bagging on Fruit Appearance and Inner Quality of Majia Pomelo
XIE Jingheng, YANG Li, DAN Shihao, QIU Li, ZHANG Wangni, LIU Dechun, HU Wei, LIU Yong
2021, 35 (1): 229-237. DOI: 10.11869/j.issn.100-8551.2021.01.0229
Abstract234)   HTML9)    PDF (4018KB)(555)      

To improve fruit quality of Majia pomelo, a characteristic cultivar in Jiangxi province, the effects of 5 types of bags and 4 debagging times on fruit appearance and inner quality of Majia pomelo were explored. The five types of bags include single-layer yellow paper bag (T1), double-layer paper bag with the outside bag being yellow and the inner bag being black (T2), three-layer paper bag with the outside bag being yellow and the inner bag being black (T3), double-layer paper bag with the outside bag being red and the inner bag being black (T4), and three-layer paper bag with the outside bag being red and the inner bag being black (T5). The four debagging times were September 25 (Z1), October 10 (Z2), October 25 (Z3), and November 10 (Z4). The results showed that the fruit appearance quality of Majia pomelo bagged with two or three layers of paper bags (CCI value: -0.38 to -0.08) was significantly higher than that without bagging and single-layer paper bags (CCI value: -1.69 to -1.40), but there was little difference in the effect of different colors on quality. Bagging had no significant effect on the fruit shape, edible rate and main fruit inclutions (total sugar, titratable acid and Vc, etc) of Majia pomelo, but T2, T3, T4 had better effect on increasing the content of main carotenoid. Among them, the fruits of T3 had the highest β-carotene content (22.64 μg·g-1), and the fruits of T2 had the highest lycopene content (29.06 μg·g-1). The experimental results of different debagging times showed that the fruit shape, edible rate and main fruit inclutions of Majia pomelo was not significantly changed, but the color of peel and flesh and the content of main carotenoid were affected. The fruits of Z3 had the best peel color (CCI value was 26.44), and the contents of β-carotene (23.17 μg·g-1) and lycopene (29.14 μg·g-1) were the highest. However, there was no significant difference with harvesting with bags. Considering the labor force, harvesting with bags after bagging was prefect. The results provided theoretical basis for improving fruit quality of Majia pomelo.

Effect of Stabiliser on the Textural Property of Egg Curd Prepared From Liquid Egg White
TANG Honggang, GUO Shichun, YANG Huijuan, XIAO Chaogeng, LU Wenjing, CHEN Di, CHEN Lihong
2020, 34 (2): 307-314. DOI: 10.11869/j.issn.100-8551.2020.02.0307
Abstract235)   HTML4)    PDF (1307KB)(695)      
In order to explore the effect of stabiliser on the textural properties of egg curd prepared from liquid egg white, sensory score and texture characteristics (hardness, gumminess, chewiness, springiness, cohesiveness and resilience) were compared for the addition of water, NaCl and stabiliser. The results showed that the sensory score and texture parameters of egg curd changed significantly with the addition of water and NaCl. The quality of egg curd was relatively better with 12% water and 1.5% salt addition. Compared with the control group, the sensory score for the test group with addition of phosphate compound, glutamine transaminase, guar gum and gellan gum was increased, and the texture was also improved. The optimum addition of the stabiliser was: compound phosphate 0.20%, TG enzyme 0.25%, guar gum 0.25%, gellan gum 0.10%. This study could provide a technical guidance for improving the processing, sensory quality and textural properties of egg curd prepared from liquid egg white.
Research Progress of Cadmium in Shellfish
FANG Ling,MA Haixia,LI Laihao,YANG Xianqing,RONG Hui,CEN Jianwei
2019, 33 (7): 1408-1414. DOI: 10.11869/j.issn.100-8551.2019.07.1408
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Cadmium is a chemical pollutant, which could do harm to the internal organs and physiological systems of human’s body by long?term exposure. Cadmium pollution is increasing seriously in shellfish with the development of industry and agriculture. This review covers the current status and toxicity of cadmium, cadmium contamination and bioaccumulation in shellfish, the commonly?used methods for the detection of cadmium. Meanwhile, the security problems of cadmium residue in shellfish were also discussed and the risk of cadmium in shellfish was analyzed and evaluated by the method of risk assessment, which would provide technological references for future studies on the quality and safety of shellfish.
Development of a Indica Rice Restorer Line Fuhui838 and Its Derivative Lines with Strong Restoring Ability and Their Utilization
DENG Da-Sheng, CHEN Hao, DENG Wen-Min, FENG Lei, YANG Xue-Feng, MA An-Lu, LIU Ming-Hai
2009, 23 (2): 175-179. DOI: 10.11869/hnxb.2009.02.0175
Abstract2005)      PDF (322KB)(1898)      

Fuhui838 and its derivative restorer lines(Fuhui718, Fuhui305, Zhongfu218, Mianhui3728, Nuoyou1 …)have the ability of strong restoring, high combining, and good resisting, which were bred and released by combined techniques of radiation-induced mutation, polymerization hybridization, and temperature tolerance screening. At present, about 43 combinations were registered and released to commercial production, the series of combinations present high seed setting rate, good tolerance to chilling and heat stress, wide adaptability, high and stable yield, the accumulated area added up to 40 million hm2. II you 838, which is famous one of these combinations, proved to be the best in long term and wide planting after Sanyou 63, and it is also one of the main hybrid rice for exporting in China.

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