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The Biological Effect of 60Co-γ Ray Radiation on Medicago polymorpha L. Seeds
CHEN Limin, LI Daxu, BAI Shiqie, ZHANG Yu, MAO Decai, LIAO Xingyong, YANG Xiaoling
2022, 36 (9): 1701-1706. DOI: 10.11869/j.issn.100-8551.2022.09.1701
Abstract66)   HTML338)    PDF (6784KB)(75)      

In order to explore biological effects of 60Co-γ ray radiation on Medicago polymorpha seeds, in this study, dried seeds of Medicago polymorpha were treated with different doses (0, 400, 600, 800, 1 000 and 1 200 Gy). The germination rate, plant survival rate, seedling height, number of branches, number of flowers and number of pods were measured, and the semi-lethal dose (LD50) of dried seeds was calculated by linear regression equation. The results showed that radiation dose of 400 Gy could significantly increase the germination rate of Medicago polymorpha seeds, while with the increase of radiation dose(400~1 200 Gy), the germination rate decreased. The radiation dose was significantly negatively correlated with survival rate, seedling plant height and number of branches; Low dose (≤600 Gy) could promote seed germination, plant growth and pods yield of bur clover, while high dose (>600 Gy) inhibited growth and fruiting of Medicago polymorpha seeds; and the semi-lethal dose of radiation to dried seeds of Medicago polymorpha was 580 Gy. This research provides a reference for mutation breeding of Medicago polymorpha.

Identification of Cassava SBT Gene Family and Function Analysis of MeSDD1
XIAO Liang, BAO Ruxue, CAO Sheng, LU Liuying, SHANG Xiaohong, ZENG Wendan, NIE Xuanhong, YAN Huabing
2022, 36 (7): 1308-1317. DOI: 10.11869/j.issn.100-8551.2022.07.1308
Abstract67)   HTML10)    PDF (6639KB)(64)      

The subtilase (SBT) gene family is an important gene family that controls plant growth and development and responds to environmental stress. In order to understand furtherly the number, basic characteristics, evolutionary relationship of SBT gene family in cassava, the SBT gene family was identified by bioinformatics, and the function of one member of the SBT gene family was characterized. The results showed that a total of 69 SBT members were identified in cassava genome, of which Manes.18G044300 was named MeSDD1 because it is most closely related to Arabidopsis. thaliana SDD1. Of all the SBT members, twenty-two SBT members had no introns, and 69 SBT members carried five highly conserved domains. Some SBT members were tandemly located on chromosomes, and drought response elements were found in their promoters. Two homozygous lines with high expression were screened out by fluorescence quantitative PCR for the subsequent experiments. The results indicated that the relative water contents in leaves of the two transgenic line were significantly higher than that of the wild type (WT), detached leaves of the transgenic lines lost water more solwly than that of WT, and the stomatal density of transgenic lines was significantly lower than that of WT. After water-deficit treatment for 14 d, the wilting degree of transgenic lines was less than that of WT. All the results indicated that MeSDD1 could enhance the drought resistance of A. thaliana. This study will provide gene resources for drought resistance breeding of cassava, and lay a theoretical foundation for the subsequently studying on the molecular mechanism of MeSDD1-mediated drought resistance.

iTRAQ-Based Proteomic Analysis Reveal Proteins Responsive to Cucumber Green Mottle Mosaic Virus Infection in Watermelon
ZHANG Huiqing, SUN Yuyan, NA Dongchen, FAN Min
2021, 35 (3): 535-548. DOI: 10.11869/j.issn.100-8551.2021.03.0535
Abstract146)   HTML5)    PDF (5288KB)(412)      
Cucumber green mottle mosaic virus (CGMMV) is a quarantine disease that affects watermelon production. To investigate the CGMMV-associated defense response in watermelon at proteomic level, we sampled watermelon leaves before and post CGMMV inoculation and employed isobaric tag for relative and absolute quantification (iTRAQ) technology to identify the differentially expressed proteins (DEPs), and KEGG analysis was carried out for these DEPs. The protein-protein interaction (PPI) networks were constructed using Cytoscape software and RT-qPCR analysis were carried out for determining the expression patterns of proteins in response to CGMMV stress. In total, we quantified 61 proteins that differentially expressed in response to CGMMV stress. These differentially expressed proteins (DEPs) included ribosomal proteins, PSI and PSⅡ proteins, ATP synthase, NADH dehydrogenase, HSP22.8, eukaryotic translation initiation factor, sucrose synthase and ethylene response factor. They were involved in KEGG pathways of photosynthesis, biosynthesis of scondary metabolites, and plant hormone signal transduction. PPI analysis identified 13, 32 and 43 DEPs for 48 h_0 h, 25 d_0 h and 25 d_48 h that involved in the known PPI network under CGMMV stress. RT-qPCR analysis obtained the expression patterns of SUS4, RPL22, NAD7, PSBH, defensin-like protein 5, ATPF, LSP1 and HSP22.8 at different infection stages of CGMMV. These results identify the proteins’ responsive to CGMMV infection, and lay a foundation for revealing molecular mechanism of CGMMV resistance in watermelon.
EMS Mutagenesis of Sweet Sorghum to Create New Germplasm
WANG Wei, CHEN Chen, GE Yubin, LUO Junjie, YE Chunlei
2022, 36 (1): 7-13. DOI: 10.11869/j.issn.100-8551.2022.01.0007
Abstract161)   HTML10)    PDF (1242KB)(123)      

In order to create and develop new sweet sorghum germplasms, sweet sorghum inbred line Tian 94 was used as donor material, and the seeds were mutated with ethyl methyl sulfonate (EMS) by soaking. The results showed that with the semi-lethality rate as the selection criterion, the optimized concentration and time of EMS treatment was 0.20%/16h; 18 materials were obtained in M5 generation by observation, identification and screening generation after generation, which were obviously different from the control in these traits such as growth period, plant height, sugar content, and so on. Among the 18 germplasms, there were 2 early maturing materials, 4 materials having high sugar content, 6 dwarf materials, 3 materials showing white grain, and 3 materials showed scattered panicle; These five type mutants were selected for, results showed that the sugar, moisture and crude fat content of these materials were all higher than those of the control. The results of this study provide new germplasms and basic materials for the genetic improvement and functional gene research of sweet sorghum, and also provide reference for mutation breeding of sweet sorghum with EMS.

Effect of Uniconazole on Physiological Characteristics of Peanut Seedlings Under Weak Light
SUN Xuewu, WU Zhengfeng, LI Lin, SUN Xiushan, WANG Caibin, LUO Sheng, ZHENG Yongmei, YU Tianyi, SHEN Pu
2016, 30 (6): 1204-1210. DOI: 10.11869/j.issn.100-8551.2016.06.1204
Abstract211)   HTML1)    PDF (1295KB)(318)      
In order to study the effect of uniconazole on peanut seedlings under weak light, a pot experiment was carried out in artificial climate box under weak light plant traits, root morphological characteristics, SPAD value of leaf, photosynthetic rate, transpiration rate, enzymes related to aging, and ultrastracture of chloroplast were determined after spraying uniconazole on the leaves.. The results showed that uniconazole decreased the height of main stem by 29.3% to 32.0% and the length of first branch by 33.3%, declined the dry weights of all organs and increased specific leaf weight. The percentage of roots with diameter greater than 0.5 mm were widened by uniconazole, while the total root length and surface area showed no significance changes among the three treatments. Uniconazole significantly promoted the SPAD values and photosynthetic rate of leaves, but exerted little effects on the transpiration rate. The activity of POD was increased by 27.3-74.0% after the use of uniconazole, whereas other enzymes related to aging (SOD, CAT) and MDA showed no significant changes. Spraying uniconazole was an effective way to increase the number of chloroplasts, grana, granum lamellae of peanut leaf, which was the physiological basis for improving photosynthetic capacity of peanut seedling under weak light. The research provides scientific basis for the use of uniconazole on the peanut relay-cropped with wheat.
Application of Electron Beam Irradiation in Life Sciences
WANG Liangyan, HONG Qihua, SUN Zhiming, HUA Yuejin
2018, 32 (2): 283-290. DOI: 10.11869/j.issn.100-8551.2018.02.0283
Abstract201)   HTML2)    PDF (1172KB)(742)      
Nowadays, irradiation treatment techniques have been widely used in various fields of life sciences. This paper focuses on its applications in fields such as food processing and preservation, medicine and health, mutation breeding, quarantine, degradation of chemical residues and allergens, aiming to promote the application of electron beam irradiation technology in life sciences, accelerate the development of processing irradiation industry to gain better economic and social benefits.
BREEDING OF NEW VARIETY YANGFUMAI 4 WITH HIGH RESISTANCE TO
WHEAT YELLOW MOSAIC DISEASE
HE Zhen-Tian, CHEN Xiu-Lan, ZHANG Rong, WANG Jian-Hua, WANG Jin-Rong, LIU Jian
2011, 25 (1): 75-78. DOI: 10.11869/hnxb.2011.01.0075
Abstract1776)      PDF (125KB)(1391)      

To control the infection of wheat yellow mosaic disease, new wheat variety with high-yield, disease-resistant was selected. Ningmai 9, which carries yellow mosaic disease resistant genes, was used as original material. Combination of conventional breeding technique and radiation methods, a new wheat variety Yangfumai 4 was developed during 1996-2007, and registered in 2008. The new wheat variety with high yield and resistance to yellow mosaic disease is suitable to plant in the Yangtze River region.

Current Status and Development Trend of Freeze Drying Technology and Industry
BI Jinfeng, FENG Shuhan, JIN Xin, YI Jianyong, LI Xuan, LYU Jian, WU Xinye
2022, 36 (2): 414-421. DOI: 10.11869/j.issn.100-8551.2022.02.0414
Abstract115)   HTML6)    PDF (1256KB)(121)      

With the continuous development of economy and science technology in China, freeze-drying (FD) technology, as an efficient drying technology that can effectively maintain the cellular structure, functional components, color, flavor, and nutrition of food, has received great attention from the food industry. In recent years, FD has been rapidly and widely applied in the precise design and manufacture of nutritious and healthy foods. This article summarizes the history, current situation as well as development trend of FD technology, equipment and products from the view food technology and industry, which may provide references for its potential application in food industry.

Development and Application of HRM Molecular Markers in Sweetpotato Based on Reduced-Representation Sequencing
FENG Junyan, LANG Tao, ZHANG Cong, LI Ming, QING Lifang, QU Huijuan, PU Zhigang, KANG Le
2022, 36 (7): 1350-1361. DOI: 10.11869/j.issn.100-8551.2022.07.1350
Abstract55)   HTML5)    PDF (6662KB)(68)      

There are few reports on the development and application of SNP markers in sweetpotato. In this study, we developed SNP molecular markers of HRM (high resolution melt) using sweetpotato reduced-representation sequencing data, and verified in sweetpotato germplasm. By analyzing sequence of 23 sweetpotato germplasm, a total of 835 756 SNP loci were found, 3 650 high-quality reads containing SNP polymorphic loci were selected, and 134 of primers were successfully developed. The results showed that 22 pairs of primers(16.42%) had no amplicons, and 15 primers(11.19%) had more than two amplified bands. The amplification products of 36 pairs of primers had no significant differences. 61 pairs of primers(45.52%) had specific amplification products, and differed among germplasm. Finally, 34 pairs of primers with rich amplification polymorphism were selected to amplify 52 sweetpotato germplasm. The results indicated that the polymorphism of molecular markers among germplasm ranged from 27.27% to 90.91%, and the average polymorphism was 59.35%. Cluster analysis showed that there were little differences among 52 sweetpotato accessions. Most bred varieties were close to the backbone parents Nancy Hall and Xushu 18. In contrast, there were great differences between the exotic sweetpotato germplasm and landraces. This study preliminarily established a new idea of developing sweetpotato SNP molecules markers based on reduced-representation sequencing technology and HRM technology, which provided a reference for the development of sweetpotato SNP molecular markers in the future. Furthermore, the molecular markers developed in this study enriched the types of molecular markers and provided support for sweetpotato rapid and efficient molecular marker study for sweet potato in the future.

Evaluation of Soil Nutrient Content and Its Distribution of Cultivated Land in South of Xinjiang: Taking Aksu Prefecture as an Example
TIAN Liwen, QI Yongchun, DAI Lu, CUI Jianping, GUO Rensong, XU Haijiang, LIN Tao, ZHANG Na
2020, 34 (1): 214-223. DOI: 10.11869/j.issn.100-8551.2020.01.0214
Abstract434)   HTML9)    PDF (2491KB)(864)      
To explore the soil nutrient content and distribution characteristics of cultivated land in south Xinjiang after years of cultivating since the second national land survey in 1982, the Aksu prefecture of xinjiang cotton producing area with typical natural ecological conditions in southern Xinjiang was taken as a representative in our research. Field investigation and laboratory analysis were used to measure the soil major element content of organic matter, total nitrogen, available nitrogen, available phosphorus, available potassium and microelement content of available copper, iron, zinc, manganese, then the determination results were analyzed by combining with relevant indices of soil nutrient grading in Xinjiang. The results showed that compared with 1982, soil nutrient content in the cultivated land was greatly improved. The average organic matter content of the whole region was 13.56 g·kg -1; total nitrogen alkaline hydrolysis nitrogen, available phosphorus and available potassium were 680, 62.30, 13.90 and 145.00 mg·kg -1, respectively. Among them, the organic matter content in Aksu Prefecture was the same as that of in 1982, while the available potassium content was decreased at rate of 2.20 mg·kg -1 annually , and the content of available nitrogen and available phosphorus were higher 74.50% and 385.86% than that of in 1982, respectively. The overall result was as follows: that the content of phosphorus and potassium was abundant, the organic matter was in the middle level, except for the effective manganese content in the lower-middle level; the content of effective copper, available iron and zinc were in different degree of abundant state, and the content of nitrogen was low. In summary, it was recommended to strengthen the implementation of the balanced fertilization program for the current nutrient content levels and trends of soil, and to improve the content of soluble fertilizers such as nitrogen through reasonable irrigation, and prevent soil salinization. Thus, this research provided a theoretical basis for the future agricultural production of soil fertility and scientific fertilization in Aksu prefecture.
Effects of Paclobutrazol and Ethephon on the Panicle Neck, Panicle Traits and Grain Filling of Foxtail Millet
YU Bingxing, WANG Hongfu, YANG Jing, DUAN Hongkai, WANG Yuyun, LI Zhi
2019, 33 (6): 1199-1207. DOI: 10.11869/j.issn.100-8551.2019.06.1199
Abstract283)   HTML3)    PDF (4038KB)(438)      
In order to explore the best treatment of paclobutrazol and ethephon which can inhibit the greedy and excessive growth of plants without affecting the yield of foxtail millet, the Nongda 8 was used as the experimental material, and the randomized block design was adopted. Paclobutrazol and ethephon were sprayed on the leaves of foxtail millet at different growth stages, respectively, and the effects of single application[Paclobutrazol was sprayed befor jointing stage(A1), jointing stage(A2) and booting stage(A3), respectively. Ethephon was sprayed at booting stage(B1), heading stage(B2) and filling stage(B3).] and interaction(Those are A1B1, A1B2,A1B3,A2B1,A2B2,A2B3,A3B1,A3B2,A3B3) of two plant growth regulators on panicle neck, panicle traits and grain filling of Nongda 8 foxtail millet were investigated. The results showed that the panicle neck diameter and dry weight per centimeter of panicle neck was significantly improved with the single treatment of paclobutrazol and ethephon, but the other parameters of the panicle neck were not significantly different. The panicle diameter was significantly increased under A1 treatment, which was 5.39% higher than CK, and the panicle weight and grain weight of panicle were the highest under B2 treatment, which increased by 7.25% and 6.94% respectively compared with CK. There was no significant difference in panicle length and panicle code number. At the same time, the grain filling rate was improved, but the blighted grain rate did not change significantly. Under the interaction of paclobutrazol and ethephon, the panicle neck diameter, bending force and dry weight per centimeter of panicle neck of millet were increased significantly, while the bend moment, height of center gravity and water content of panicle neck decreased obviously. Compared with CK, the panicle length and panicle code number of millet decreased by 20.11% and 9.75% respectively, and the panicle diameter increased by 10.15% under A1B3 treatment. The panicle weight and grain weight of panicle reached the maximum under A2B2 treatment, which increased by 11.45% and 9.50% respectively compared with CK. With the increase of grain filling rate, the blighted grain rate decreased significantly, and reached the minimum under A2B2 treatment, which decreased by 50.00% compared with CK. The results showed that the interaction effect of the two regulators was more significant than that of single application, after spraying 300 mg·L -1 paclobutrazol on the before jointing stage or jointing stage of foxtail millet, it is most suitable to pursue the application of 400 mg·L -1 ethephon in heading or filling stage, while the application of ethephon at booting stages will decrease grain weight of panicle and yield decrease. The results of this study provided a theoretical basis for chemical regulation and high-yield cultivation of foxtail millet.
SPACE RADIATION MEASUREMENT OF PLANT SEEDS BOARDING ON THE SHIJIAN-8 SATELLITE
L Dui-cai~1 HUANG Zeng-xin~1 ZHAO Ya-li~1 GUO Hui-jun~2 WANG Gen-liang~1JIA Xiang-hong~1 LI Chun-hua~3 ZHANG Long~3 LIU Lu-xiang~2(1.China Astronaut Research and Training Center,Beijing 100094;2.Institute of Crop Science,Chinese Academy of Agricultural Science/National Key Facility for Crop Gene Resources and Genetic Improvement,Beijing 100081;3.Chinese Academy of Space Technology,Beijing 100094)
2008, 22 (01): 5-8. DOI: 10.11869/hnxb.2008.01.0005
Abstract1307)      PDF (248KB)(1593)      

In order to identify cause of mutagenesis of plant seeds induced by space flight, especially to ascertain the interrelation between space radiation and mutagenesis,a "photograph location" experimental setup was designed in this study.CR-39 solid-state nuclear track detectors were used to detect space heavy particles.The plant seeds and their position hit by space heavy ions were checked based on relative position between track and seeds in the setup.The low LET part of the spectrum was also measured by thermoluminescence dosemeter(TLD,LiF).The results showed that the "photograph location" experimental method was convenient,practicable and economical.This new method also greatly saved time for microscopical analysis.On Shijian-8 satellite,the average ion flux of space heavy ions was 4.44 ions/cm~2·d and the average dosage of low LET space radiation to the plant seeds was 4.79 mGy.

Genome Wide Identification and Expression Analysis of the SBP Gene Family in Foxtail Millet
SONG jian, CAO Xiaoning, WANG Haigang, CHEN ling, WANG Junjie, QIAO Zhijun, LIU Sichen
2020, 34 (7): 1409-1420. DOI: 10.11869/j.issn.100-8551.2020.07.1409
Abstract268)   HTML2)    PDF (3178KB)(731)      
In order to further elucidate the important function of SBP gene family in foxtail millet, NCBI blast and HMMER search were used to identify the SBP genes in foxtail genome, and to analyze the physicochemical properties and phylogenetic tree of SBP family in foxtail millet. The expression SBP gene family in foxtail millet treated with PEG and ABA were detected by real-time fluorescence quantitative PCR (RT-qPCR). The results showed that 19 members of SBP gene family were identified from the whole genome of foxtail millet and they encoded proteins with 199-1 118 aa, which distributed across 9 chromosomes. The results of phylogenetic tree showed that the SBP protein could be divided into 2 categories. The first category was classified into 6 sub-categories, and the second category only contained one SBP proteins. The numbers of exons were variable, ranged from 2-11, and all of the SBP proteins were hydrophilic protein. The results of subcellular localization prediction showed that the members of the gene family were located mainly in the nucleus except SBP7. Cluster analysis showed that foxtail millet SBP was closely related to monocotyledonous sorghum and rice than to cotyledonous Arabidopsis thaliana. Upstream promoter region analysis founded a large number of cis-acting elements closely related to hormones, drough, light, pathogenic factors and stress condition. The results of RT-qPCR showed that the relative expression of SBP gene was up-regulated under PEG and ABA treatment, and the expression quantity of SBP in leaf was higher than that in stem. The expression quantity of SBP under PEG was significantly higher than ABA treatment. The expression levels of SiSBP13 in leaves and stems after 4 hours of PEG stress were 180 and 15 times as that before treatment. After 4 hours of ABA induction, the expression of SiSBP13 in stems was up to 8 times that before stress. After 24 hours of induction, the expression of SiSBP13 in leaves was 28 times higher than that before stress. This study laid an important foundation for further analysis of the specific functions of SBP genes at different development stages in foxtail millet.
Collocation Modes for Different Varieties of Double-Cropping Direct-Seeding Rice
LI Zujun, HE Xunfeng, TAN Yiqing, ZENG Yanhua, ZENG Yongjun, SHI Qinghua, WU Ziming, LI Huijie
2021, 35 (3): 688-696. DOI: 10.11869/j.issn.100-8551.2021.03.0688
Abstract148)   HTML5)    PDF (1791KB)(362)      
In order to investigate the effects of different early and late rice combinations on growth characteristics, yield formation and rice quality under double-cropping and direct seeding conditions, three early rice varieties (QLY2012, TLY 83, XZX45) and two late rice varieties (YY1538 and TY398) were used as experimental materials in this experiment. Four combination modes were designed, inbred indica rice XZX45 planted as control treatment (CK): XZX45-XZX45; Early indica-Late japonica (M1): QLY2012-YY1538; Early Indica-Late Indica (M2 same variety planted as early rice and late rice): QLY2012 - QLY 2012; Early Indica and Late Indica (M3 different varieties planted as early rice and late rice): TLY83 - TY398, with control (CK): XZX45-XZX45. The annual growth period, population quality, yield composition, annual yield, rice quality and RVA characteristic spectrum of rice starch were studied. The results showed that, these combinations were all heading safely, and the longest annual growth period was M1 (222 days), which was 16 days longer than CK; the highest annual total yield was M1 (14.82 t·hm -2), which increased 1.56 t·hm -2 compared with CK, with an increase rate of 11.76%; the second was M2, its annual total yield was 14.29 t·hm -2, which increased by 1.03 t·hm -2 and the growth rate was 7.77% compared with CK, and the annual total yield of M3 increased by 5.28% compared with CK. The analysis on the characteristics of population quality and yield components of different early and late rice collocation modes in double-cropping and direct-seeding rice, showed that the collocation mode with higher annual yield had the characteristics of large dry matter accumulation from heading stage to maturity stage, large leaf area index from booting stage to maturity stage, large spike size, high seed setting rate and daily yield of early and late rice. M1, M2 and M3 treatments had better rice quality and RVA profile than CK, but no significant difference was observed in early rice. Generally speaking, the collocation mode (M1) guarantees annual yield, and rice quality is better than other modes, which can provide a reference for portable cultivation of double-cropping rice.
Effects of Acetosalicylic Acid on Fucoxanthin Content in Phaeodactylum tricornutum and Its' Molecular Mechanism
ZHANG Li, GONG Yifu, ZHU Shuaiqi, LIU Hao, LI Shenrui, XIE Zhihao, WANG Heyu
2020, 34 (7): 1432-1439. DOI: 10.11869/j.issn.100-8551.2020.07.1432
Abstract223)   HTML1)    PDF (1430KB)(442)      
The objective of this research is to investigate the effects of acetosalicylic acid (ASA) on cell growth, fucoxanthin accumulation and expression levels of genes related to the biosynthesis of fucoxanthin in the model diatom Phaeodactylum tricornutum. Growth rate, fucoxanthin content and gene expression were detected by spectrophotometer, high performance liquid chromatography (HPLC) and real-time PCR respectively. The results indicated that fucoxanthin content were closely associated with the concentrations of ASA. ASA at a certain concentration suppressed proliferation of algae cells. With the concentration of ASA iuncreased, the content of fucxanthin was first increased and then declined. Notably, the content of fucoxanthin in Phaeodactylum tricornutum treated with 10 mg·L -1 ASA was the highest (1.78 g·kg -1DW), which was 1.9 times as much as that in control groups. The expression of genes (including PSY, PSD, ZDS, CRTISO, LCYB and ZEP) that encoding pivotal enzymes associated with the fucoxanthin biosynthesis were increased following ASA treatment. In particularly, the transcripts of detected genes were significantly up-regulated under 10 mg·L -1 ASA treatment when compared with the control ( P<0.01). Principal component analysis (PCA) indicated these six genes were positively correlated with fucoxanthin accumulation, therein ZEP exhibited the highest contribution. In general, proper concentrations of ASA induced the expression of genes responsible for fucoxanthin biosynthesis and increased the production of fucoxanthin. The present study provides a theoretic basis for molecular mechanism of fucoxanthin biosynthesis in Phaeodactylum tricornutum.
Transcriptome and Jasmin Metabolism Gene Analysis of Passiflora edulia Sims Under Low Temperature Stress
WEI Xiaoxia, WANG Xiao'an, CHEN Jin, LAI Ruilian, WU Rujian
2021, 35 (4): 815-825. DOI: 10.11869/j.issn.100-8551.2021.04.0815
Abstract181)   HTML4)    PDF (5858KB)(550)      
To study the response mechanism of Passiflora edulia Sims ( P. edulia) to low temperature stress, P. edulia plants treated under 0℃ and 25℃ were used as materials for transcriptome sequencing and digging out genes related jasmonic acid (JA) metabolism on Illumina HiSeq sequencing platform. A total of 45.30 Gb clean data were obtained, and 39 521 unigenes and 5 311 different expression genes (DEGs) were assembled. According to GO classification, these DEGs were classed into cell component, molecular function and biological process. Among them, metabolic pathways in biological process contained most DEGs, including sterol biosynthesis, flavonoid lipidization, tyrosine metabolism, L-phenylalanine biosynthesis, cork-fat biosynthesis, glucosinolide metabolism and long-chain aliphatic acyl coenzyme A metabolism. KEGG pathway enrichment analysis showed that ribosome pathway, starch and sucrose metabolism pathway, plant hormone signal transduction pathway, plant and pathogen interaction pathway were important metabolic pathways of P. edulia in response to low temperature stress. Based on qRT-PCR, AOC, AOS, JAR1, MYC2, PYL and JAZ, the key genes of JA metabolism pathway in P. edulia were all up-regulated under low temperature stress while COI1 was down-regulated. The validation results were similar to the trends of FPKM values obtained by sequencing, indicating that the sequencing results were relatively accurate. Our research indicated that the response mechanism of JA to low temperature stress in P. edulia was basically consistent with that in model plants, but the regulation of genes such as COI1 and MYC2 needed further functional verification. These results would provide scientific references for further research on cold resistant mechanism of P. edulia.
Effects of Different Drying Temperatures on the Nutritional Quality and Germination Characteristics of Peanut After Harvest
DU Qi, ZHAO Xinhua, GENG Leiyue, ZOU Tuo, ZUO Yongmei, LI Xinming, ZHANG Wei, YU Haiqiu
2020, 34 (10): 2254-2260. DOI: 10.11869/j.issn.100-8551.2020.10.2254
Abstract167)   HTML4)    PDF (2389KB)(379)      
To screen out the suitable drying temperature for different peanuts, HY22 (large-size), HY20 (medium-size) and NH16 (small-size) were used to evaluate the quality and germination parameters at different drying temperatures. The results showed that the quality of HY22, HY20 and NH16 were decreased due to the increased acid value and peroxide value with the increased temperature, but no effect on the content of protein, oleic acid, linoleic acid and amino acid. When the drying temperature was 40℃, the electrical conductivity of HY22, HY20 and NH16 were 2.57, 1.85 and 3.38-fold compared to the controls after soaking of 12 hours, respectively. There were no significant differences in HY22, HY20 and NH16 of germination potential, germination rate, germ length, and germ weight. When the drying temperature was 50℃, the electrical conductivity of HY22, HY20 and NH16 were 2.4, 1.61 and 3.12-fold after soaking of 12 hours, respectively. The germination potential and rate of HY22 were significantly decreased by 24.30 and 6.25 percentage points, and, germ length and weight were significantly decreased by 25.21% and 24.62%. The germination potential of NH16 was significantly by 16.25 percentage points, and germ weight was significantly decreased by 22.14%. But there was no significant change in HY20. When the drying temperature was higher than 50℃, the germination rates of HY22, HY20 and NH16 were significantly decreased. These results showed that high-temperature drying could decrease the quality and germination performance of peanuts. This results study could provide a theoretical basis for the rapid drying technology of peanuts after harvest.
Effects of Zinc and Boron Rate on Ear-kernel Traits and Grain Quality in Maize
ZHANG Yongqiang, SONG Hang, XUE Zhiwei, NIU Pingping, JIE Lingjun, LI Chaohai
2017, 31 (2): 371-378. DOI: 10.11869/j.issn.100-8551.2017.02.0371
Abstract209)   HTML1)    PDF (1232KB)(395)      
In order to explore the effects of zinc and boron on ear traits of maize, a pot experiment was conducted with four treatments (application without zinc and boron, application of zinc, application of boron, application of zinc and boron), using a hybrid Yudan 606 and the changes of ear-kernel traits and grain quality were investigated. The results showed that application of zinc or boron could obviously reduce bare tip length and increase kernels per row and yields. In terms of the kernel traits, applying zinc fertilizer increased kernel length, but decreased kernel thickness,100-kernel volume and 100-kernel weight. The grain yield increased due to the increased kernel numbers per ear under the condition of applying zinc fertilizer alone. In contrast, kernel length,100-kernel weight,100-kernel volume and single ear yield were increased when applied boron alone. Quality characters of a grain were analyzed, and the results showed as follows: compared with CK, applying zinc fertilizer or boron fertilizer increased protein concentration in grain. Applying zinc fertilizer could significantly increase the content of fat, but applying boron fertilizer make it lower. Furthermore, there was no significant change on starch content. Compare with CK, application of zinc fertilizer or boron fertilizer also significantly increased zinc and boron content in grain. This study provides a theoretical basis for the application of zinc and boron in the production of maize.
Comprehensive Evaluation of Drought Resistance at Seedling Stage in Common Bean( Phaseolus vulgaris L.)Material
LI Jie, ZHANG Xiaoning, JIN Fansheng, HAN Yanlong, LI Haijin
2022, 36 (8): 1516-1529. DOI: 10.11869/j.issn.100-8551.2022.08.1516
Abstract55)   HTML118)    PDF (1887KB)(45)      

The producing areas of common beans in China was mainly distributed in arid and semi-arid region. Drought is one of the major limiting factors for common beans' production. In this study, two treatments (drought stress and normal water supply) were employed to evaluate the drought resistance of fifty common bean cultivates in pot experiments. The morphological and physiological indexes were determined at 12 days after drought treatment and 3 days after rewatering. A method of combining drought resistance comprehensive evaluation value (D value), clustering analysis, stepwise regression analysis and path analysis were applied to perform the drought resistance identification and drought resistance index selection of common beans at seedling stage. The result showed that the D value were significantly correlated with SOD and POD activity of 12 days after drought treatment and the relative content of chlorophyll, leaf relative water of 3 days after rewatering, and the D value were extremely significantly correlated with the content of MDA of both drought treatment and rewatering. Clustering analysis according to D value can divide 50 materials into different drought resistance grades, the coincidence rate of the two classification results was 40%, the adjacent grade rate was 60%, and there was no cross-grade material. The 50 common beans materials were divided into 5 groups,in which ten varieties were high resistance, twenty-five were medium and high resistance, seven were moderate resistance, six were medium and low resistance and two were low resistance. In conclusion, the combination of D value of drought treatment and rewatering could improve the reliability of drought resistance identification. SOD activity, POD activity, MDA content of drought treatment and the relative content of chlorophyll, MDA content, leaf relative water of 3 days after rewatering could be used as the indexes for indentifing common bean drought resistance identification at seedling stage, MDA content of the two treatments could be directly used in drought resistance identification of common bean resources. The results provide a theoretical reference and method for simplifying the drought resistance indentification of common beam resources.

Genome-wide Identification of KNOX Gene Family in Tomatoand Their Evolutionary Relationship in Solanaceae
YE Shuguang, ZAI Wenshan, XIONG Zili, ZHANG Haili, MA Yanru
2017, 31 (7): 1263-1271. DOI: 10.11869/j.issn.100-8551.2017.07.1263
Abstract318)   HTML10)    PDF (7547KB)(431)      
To investigate the characteristics of KNOX gene family in tomato and their evolutionary relationships in Solanaeae, KNOX gene family in tomato and other Solanaceae (potato and pepper) were identified using bioinformatics method. The results showed that the tomato genome contained 8 SlKNOX genes ( SlKNOX1- SlKNOX8), distributed across 8 chromosomes of tomato. Multiple sequence alignment showed, except for SlKNOX5, all other proteins included 4 conservative domains (KNOX1, KNOX2, ELK and Homeobox KN), which were specific for plant KNOX proteins. Phylogenetic analysis characterized 2 classes of KNOX genes: Class I with 3 sub-classes (A, B and C) and Class II with 2 sub-classes (A and B). Compared with KNOX genes in Arabidopsis, KNOX genes in Solanaceae have a different evolutionary process. Furthermore, a novel branch (Class IC) has occurred in Solanaceae. qRT-PCR analysis revealed SlKNOX1 was high expression in bud and flower, gradually reduced in the process of fruit mature. Expression of SlKNOX2, SlKNOX3 and SlKNOX4 was not deleted in the process of fruit mature. SlKNOX6 and SlKNOX7 were expressed in all tested tissues. Expression level of SlKNOX8 was gradually enhanced in the process of fruit mature, and the expression in root was the highest. In this study, the characteristics of KNOX gene family in tomato were revealed on the genome level and their phylogenetic relationships in Solanaceae were clarified, which will contribute to further elaborate their functions in different tissues and their evolutionary relationships in Solanaceae.
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