%0 Journal Article %A TANG Jie %A CHEN Zhiqing %A GUO Annan %A QIU Qiongfen %T Characteristics of Microbial Community Structure in the Rhizosphere Soil of Different Crops %D 2021 %R 10.11869/j.issn.100-8551.2021.12.2830 %J Journal of Nuclear Agricultural Sciences %P 2830-2840 %V 35 %N 12 %X

The Rhizobacteria plays a crucial role in plant growth, development and health. In this study, rhizosphere soils of seven different crops, including soybean [Glycine max (L.) Merr.], maize (Zea mays), peanut (Arachis hypogaea L.), kidney bean (Phaseolus vulgaris L.), cowpea [Vigna unguiculata (L.) Walp], sweet potato [Ipomoea batatas (L.) Lam.] and cocoyam [Colocasia esculenta (L.) Schoot], were collected in July 2017 to explore the effects of crop taxa on rhizosphere microbiomes. Illumina MiSeq sequencing and phospholipid fatty acid (PLFA) were used to investigate the microbial community structure and diversity of rhizosphere soil. The difference in microbial PLFA biomarker contents among different crops were observable. Compared with the other crops, the total PLFAs and the biomass ratio of fungi and bacteria (F/B) in the rhizosphere soil of peanut were the highest, whereas the biomass ratio of gram positive bacteria and gram negative bacteria (G+/G-) was the lowest. Although the seven groups had similar dominant phyla, such as Proteobacteria, Actinobacteria, Acidobacteria and Firmicutes, their bacterial compositions at the class and order level were obviously different. Alpha diversity analysis showed that the OTU richness (Chao1, P <0.001) and bacterial community diversity (Shannon, P <0.001) in the rhizosphere of soybean was the highest among the seven crops. Non-metric multidimensional scale analysis (NMDS) revealed that the rhizosphere microbial community structure formed significant clustering with different crops under OTU and PLFAs levels. The screening and comparison of sensitive microorganisms in the rhizosphere further showed that crops had distinctive selection of the rhizosphere bacterial community and species-sensitive microorganism. These results indicated that the rhizosphere microbiome was correlated with crop taxa. This study can provide a basis on which to construct a healthy plant rhizosphere microbiome to improve plant breeding.

%U https://www.hnxb.org.cn/EN/10.11869/j.issn.100-8551.2021.12.2830