%0 Journal Article %A ZHOU Qian %A CHEN Yun %A WANG Yuzhou %A WANG Jilian %A KAIDIRIYE· %A Yusupul %A ZHAO Huixin %T Expression Response to Low-Temperature Stress and Codon Bias Analysis of LaBBX Gene in Lepidium apetalum %D 2021 %R 10.11869/j.issn.100-8551.2021.06.1253 %J Journal of Nuclear Agricultural Sciences %P 1253-1262 %V 35 %N 6 %X BBX is a kind of important transcription factors which belongs to zinc finger proteins in plants. BBX regulate the expression of some genes involved in abiotic stress, and improve the resistance of plants to adversity stress. In order to explore the expression pattern of LaBBX gene responds to low temperature stress in Lepidium apetalum seedlings and analyse its codon bias. Based on the previous analysis of Lepidium apetalum transcriptone database BBX CDS sequence was cloned. The cDNA of this gene was 733 bp in length, encoding 242 amino acids, and was named as LaBBX. LaBBX contained two B-box zinc finger protein domains, its protein molecular weight was about 61.66 kDa and the theoretical isoelectric point was 5.09, with no signal peptide segments and no transmembrane region. The qRT-PCR analysis showed that LaBBX gene was rapidly and significantly up-regulated when seedlings of Lepidium apetalum were under low temperature stress, after 6 hours stress, the expression of LaBBX was 21 times as the control, and retained high level expression within 24 hours. The results suggest that LaBBX play an important role in regulating the resistance of low temperature stress in Lepidium apetalum seedling. Analysis of codon usage bias of the Lepidium apetalum LaBBX gene showed that: The ENc, CAI, and GC content of LaBBX were respectively 55.453%, 0.244%, and 46.78%, indicating that the codon bias level of LaBBX in Lepidium apetalum was low and biased toward the synonymous codons with A or T. According to the RSCU value, 8 codons showed hign-frequency. The CDS sequence and RSCU cluster analysis showed that the preference of LaBBX gene was closest to that of Brassicaceae burnett in dicotyledonous plants. Many factors play an important role in shaping codon usage bias of the Lepidium apetalum LaBBX gene such as mutation and selective pressure. The yeast expression system was found to be most suitable for heterologous expressing LaBBX gene, and Arabidopsis thaliana is the most ideal genetic transformation receptor of the LaBBX gene. This study provides important information for further studies on the heterologous expression and the function of the LaBBX gene in Lepidium apetalum. %U https://www.hnxb.org.cn/EN/10.11869/j.issn.100-8551.2021.06.1253