%0 Journal Article %A SU Wang %A HU Luhua %A WANG Jian %T Effects of Mulching on Ridge-Furrow for Harvesting Rainwater on Activity and Gene Expression of Starch Synthesis Key Enzyme and Starch Accumulation of Rain-Fed Potato Tuber %D 2021 %R 10.11869/j.issn.100-8551.2021.03.0737 %J Journal of Nuclear Agricultural Sciences %P 737-744 %V 35 %N 3 %X To investigate effect of mulching on ridge-furrow for harvesting rainwater on the activity and gene expression of starch synthesis key enzyme and starch accumulation of rain-fed potato tuber during tuber formation, using Qingshu 9 as experimental material, the cultivated patterns included whole plastic mulching on double ridges and single ridge mulching with plastic film, the bare land flat sowing was set as control. The activity and gene expression of key enzymes in starch synthesis and starch accumulation indexes were determined by Ultraviolet-Visible Spectrophotometer and Fluorescence Quantitative PCR. The results showed that, compared with the control, under the whole plastic mulching on double ridges and single ridge mulching with plastic film, the soluble starch synthase (SSS) activity was increased significantly by 77.70% and 22.63%, respectively, and SSIII gene expression was increased significantly by 32.26% and 119.35%, respectively. Meantime, the activity of ADP-glucose pyrophosphorylase (AGPP), granule-bound starch synthase (GBSS) and ADP-glucose pyrophosphorylase AGPase, soluble starch synthase SSII, granule-bound starch synthase GBSSI, starch branching enzyme SBEI, and starch branching enzyme SBEII gene expression levels showed significant decrease, but the activity of starch branching enzyme (SBE) was not affected during the whole process of potato tuber formation. Compared with the control, the total starch content, amylase content and ratio of amylose to amylopectin were increased significantly by 5.04%, 17.57% and 27.81% under the whole plastic mulching on double ridges, respectively, while the starch accumulation index was decreased significantly by single ridge mulching with plastic film. SSS enzyme activity showed a significant negative correlation with GBSSI gene expression, and a significant positive correlation with amylase content and amylose to amylopectin ratio. The other key enzyme activity showed no significant correlation with the key enzyme gene expression and the starch accumulation index. The results are of great significance for selecting production measures of potato with high quality starch in the eastern arid area of Qinghai province. %U https://www.hnxb.org.cn/EN/10.11869/j.issn.100-8551.2021.03.0737