%0 Journal Article %A DING Haomiao %A DU Haofei %A FU Ruijie %A JIN Xudong %A XIE Ce %A CHEN Tongni %A WANG Caisheng %A QIAN Guoying %T Analysis of Differentially Expresses Genes in Human Erythroleukemia Cells Induced by Sulfated Polysaccharide Fraction From Sargassum fusiforme %D 2021 %R 10.11869/j.issn.100-8551.2021.03.0595 %J Journal of Nuclear Agricultural Sciences %P 595-604 %V 35 %N 3 %X To study the systematic transcriptomic changes of human erythroleukemia cells (HEL) stimulated with polysaccharide fraction of Sargassum fusiforme (SFPS Ⅱ). HEL cells were treated with SFPS Ⅱ for 24 hours, the differentially expressed genes was analyzed by transcriptome technology. The genes were analyzed with Gene Oncology (GO) Term and Kyoto Encyclopedia of Gene and Genomes (KEGG) to screen the significantly enriched signals. The differently expressed genes were verified with the real-time quantitative PCR (RT-qPCR). After treated with SFPS Ⅱ, the cell proliferation of HEL cells were inhibited, cell viability was reduced in a concentration-dependent manner, while showed less toxic effect in normal human embryonic lung (MRC-5) cells. Compared with the control, a total of 1 248 differentially expressed genes (DEGs) were identified, including 219 up-regulated genes and 1 029 down-regulated genes. GO analysis indicated that differentially expressed genes mainly exercised in the molecular functions of DNA conformation change, sulfate transmembrane transport, response to tumor necrosis factor and regulation of extrinsic apoptotic signaling pathway. Pathway enrichment analysis showed that these genes involving in Rap 1 signaling pathway and immune response. Finally, with 16 DEGs which were randomly selected, and confirmed by RT-qPCR, the differentially expressed genes resulted from SFPS Ⅱ treating in HEL cells were significantly correlated with the biological processes and signaling pathways related to cancer. That could provide a theoretical basis for revealing the antitumor mechanism of sulfated polysaccharide from Sargassum fusiforme. %U https://www.hnxb.org.cn/EN/10.11869/j.issn.100-8551.2021.03.0595