%0 Journal Article %A DU Wenli %A CHEN Zhongshan %A XU Duanxiang %A XU Tongwei %A GAO Shan %A WEN Qingfang %T Physiological Response and Differentially Expressed Genes Analysis of Transcriptome in Momordica charantia L. Leaf Under Cold Stress %D 2021 %R 10.11869/j.issn.100-8551.2021.02.0338 %J Journal of Nuclear Agricultural Sciences %P 338-348 %V 35 %N 2 %X Low temperature during growing season affects the development and yield of bitter gourd (Momordica charantia L.), which limits the geographical locations. In order to understand tolerance mechanism to cold stress of bitter gourd, inbred line NO.43 was tested under low temperature stress in this research. The phenotype, relevant physiological and biochemical indexes, and transcriptome of No.43 were observed and determined after cold stress treatment under 8℃. The main results are as follows: inbred line NO.43 showed cold tolerance under cold stress. In addition, the content of MDA, Pro, and REC(Relative electrolyte leakage rate)in bitter gourd significant changed under cold stress. The transcriptome under cold stress (8℃) treatments with 0 and 12 h is also analyzed. The results showed that in response to cold, a total of 1 285 differentially expressed genes (DEGs) were detected, including 916 up-regulated and 369 down-regulated genes. In addition, sone metabolism pathways involving a large number of candidate genes were identified to be regulated by cold. Moreover, 10 genes related to cold stress reported from other publications were also detected, including transcription factors of WRKY, NAC, MYB families. We validated the expression profiles of 10 candidates genes by qRT-PCR,the results are consistent with the phenotypic and physiological changes, which further confirmed the accuracy of RNA-seq results. In this study, the transcriptome changes of bitter gourd under low temperature stress, as well as the molecular mechanisms of dynamic changes and physiological responses to low-temperature-resistant genes were revealed at the genome-wide level. This study provides some insights into the cold tolerance mechanism and digging out useful candidate genes for genetic improvement. %U https://www.hnxb.org.cn/EN/10.11869/j.issn.100-8551.2021.02.0338