%0 Journal Article %A LI Yongping %A YE Xinru %A WANG Bin %A CHEN Mindong %A LIU Jiangting %A ZHU Haisheng %A WEN Qingfang %T Cloning and Selection Evaluation of Reference Gene for Quantitative Real-Time PCR in Hibiscus esculentus L. %D 2021 %R 10.11869/j.issn.100-8551.2021.01.0060 %J Journal of Nuclear Agricultural Sciences %P 60-71 %V 35 %N 1 %X The study was aimed at selecting the stable reference genes to ensure the reliability and accuracy in gene expression analysis of Hibiscus esculentus L.. The ORF sequences of 18SrRNA, ACT, EF-1a, TUA, TUB and GAPDH were screened and verified(obtained) from ‘Green White No. 1’ according to the RNA-seq database of Hibiscus esculentus L.. By quartitative real-time technology, combined with GeNorm, NormFinder and BestKeeper software analysis, we evaluated the expression stability of six reference genes in different tissue, different development stages of fruit and leaf and different stress treatments (low temperature, high temperature, drought). The results showed that six genes can express in different tissues, development stages and stresses, but the expression stabilities were not the same. Among them,EF-1a was the most stable under pod development and high temperature stress. 18SrRNA was the most stable under various tissue, leaf development and drought stress. ACT was the most stable under low temperature stress. In addition, with all 29 treatments, the expression of 18SrRNA, EF-1a and ACT was relatively stable and can be used for fluorescence quantitative expression analysis. This study provides a basis for the study of gene function and regulation mechanism in Hibiscus esculentus L. %U https://www.hnxb.org.cn/EN/10.11869/j.issn.100-8551.2021.01.0060