%0 Journal Article %A GAO Qi %A LIU Ziheng %A GENG Yi %A JIN Xiaoyi %A WU Jiale %A LU Yating %A ZHANG Qian %A XUE Youlin %T Optimization of the Extraction Process of Soluble Protein From Peanut Leaf by Response Surface Methodology and Its Antioxidant Activity %D 2020 %R 10.11869/j.issn.100-8551.2020.04.0796 %J Journal of Nuclear Agricultural Sciences %P 796-804 %V 34 %N 4 %X The effects of extraction pH, solid-liquid ratio and extraction together with the alkalidissolution and acid precipitation method, time on the protein extraction rate were investigated for the extraction process of peanut soluble leaf proteins. Based on the results of single factor tests, the Box-Behnken design and response surface method (RSM) were applied to optimize the extraction rate and the antioxidant activities of peanut leaf soluble proteins were also investigated. The results showed that the order of factors affecting the extraction rate of peanut leaf proteins was pH>extraction time>solid-liquid ratio, and the optimal conditions were: pH9, liquid-solid ratio 1:20(w/v)and extraction time 40 min. The regression model obtained was highly significant (R2=0.992 7), showing excellent goodness-of-fit. The predicted peanut soluble leaf protein extraction rate was 52.8% and the actual protein extraction rate was 54.2% with 63.8% purity, which showed strong DPPH and superoxide anion radical scavenging abilities and good reducing power. The results of this study could provide a theoretical support for the development and utilization of peanut leaves and the extension of the value chain peanut industry. %U https://www.hnxb.org.cn/EN/10.11869/j.issn.100-8551.2020.04.0796