%0 Journal Article %A LAI Chengchun %A PAN Hong %A HUANG Xiangui %A FAN Lihua %A LAI Zhongxiong %A DUAN Changqing %A LIU Wenhui %T Cloning and Expression Analysis of UFGT From Spine Grape (Vitis davidii Foëx.) Callus %D 2019 %R 10.11869/j.issn.100-8551.2019.09.1677 %J Journal of Nuclear Agricultural Sciences %P 1677-1685 %V 33 %N 9 %X

In order to reveal the function of UDP-glucose: flavonoid 3-O-glucosyltransferase (UFGT) in regulating anthocyanin biosynthesis in spine grape (Vitis davidii Foëx.) callus at the cellular level, the gene encoding VdUFGT was isolated from spine grape callus by using RT-PCR with RACE technology. Its sequence was analyzed by bioinformatics and the expression profiles were investigated by real-time quantitative PCR (RT-qPCR). The results showed that the open reading frame (ORF) of cDNA and DNA of VdUFGT are 1 371 bp and 1 448 bp in length, respectively. It contains two exons and one intron, encoding 456 amino acid residues. The VdUFGT protein, an unstable and hydrophilic protein with negative charges, is a member of the UDPGT superfamily, including a UDPGT domain and a UDP: flavonoid glycosyltransferase YjiC feature regions. Six Vitis plants are clustered into the same group according to the phylogenetic tree, which constructed by proteins encoded from UFGT homologous genes. RT-qPCR analysis showed that the VdUFGT transcription level of red callus of spine grape was significantly higher than that of white callus, which was above 79 times when they were cultured for 25 days. During the continuous culture of spine grape callus, the VdUFGT expression level changed greatly in red callus, two peaks occurred at the middle stage of rapid-growth and the beginning of senescence, respectively. While the expression level of VdUFGT in white callus of spine grape changed not as wells as in red callus, it always maintained at a very low level. These results suggested that VdUFGT is the major gene involved in the regulation of anthocyanin biosynthesis in cell cultures of spine grape callus, and its regulatory role was mainly worked at the middle stage of rapid-growth and the beginning of senescence. This result would lay a foundation for further studying of the regulatory mechanism of anthocyanin biosynthesis in spine grape cells.

%U https://www.hnxb.org.cn/EN/10.11869/j.issn.100-8551.2019.09.1677