%0 Journal Article
%A YANG Xu
%A YANG Zhiling
%A TAN Mei
%A CHENG Xiaoyan
%T Characteristics Analysis of Houpoëa officinalis Transcription and Development of EST-SSR Markers
%D 2019
%R 10.11869/j.issn.100-8551.2019.07.1318
%J Journal of Nuclear Agricultural Sciences
%P 1318-1329
%V 33
%N 7
%X In order to investigate genomic information and develop molecular markers of Houpoëa officinalis, Illumina-Solexa Genome sequencing platform was used to analyze different tissues of velamen, stem bark and leaf of 9 individuals to get a large number of Unigenes, and to develop abundant SSR markers on the basis of the transcription sequencing data. The results showed that a total of 109 526 Unigenes with an average length of 1 023 bp were produced, among which 60 361, 39 942, 65 535, 51 351 and 27 310 were annotated in NR, Swiss-Prot, GO, KEGG and KOG database, respectively. A total of 25 949 sequences with 27 721 SSRs were detected through transcription of H. officinalis, SSR sites occurred with a frequency of 23.67%. Among the total of 374 identified EST-SSRs, CT/GA (31.8%) and AG/TC (27.90%) were the dominant di-nucleotide repeat motifs, AGA/TCT and AAG/TTC were the dominant tri-nucleotide repeat motifs. A total of 180 SSR markers were randomly selected for validation, among these, 104 produced reproducible amplicons and 21 were polymorphic which showed that EST-SSRs of H. officinalis were polymorphic. The results of this study laid a good foundation for genetic analysis, map construction and comparative genome of H. officinalis and related species.
%U https://www.hnxb.org.cn/EN/10.11869/j.issn.100-8551.2019.07.1318