%0 Journal Article %A WANG Xiaodan %A CHEN Hao %A ZHANG Zhenqian %A GUAN Chunyun %T Relationship Between Two Resistance Gene and Diseases in High Oleic Acid Rapeseed %D 2019 %R 10.11869/j.issn.100-8551.2019.05.0894 %J Journal of Nuclear Agricultural Sciences %P 894-901 %V 33 %N 5 %X In order to rapid screen strongly resistant high oleic acid rapeseed varieties, In this study, 20 high oleic acid rapeseed varieties were used as materials,the disease resistance of different materials was investigated,and disease-resistance related genes expression of HSP90 and ATG3 which screened from microRNA sequencing and detected by RT-qPCR, then the relationship between related genes and disease index were analyzed.The results showed that there was a significantly linear positive correlation between incidence and disease index of 20 high oleic acid rapeseed varieties,the expression of HSP90 decreased gradually until the flowering stage, and there were two kinds of expression patterns in the pod stage with Low - high - low and gradually decreased. ATG3 gene expression increased gradually until the flowering stage, and also appeared the same expression patterns with HSP90 in pod stage. When the expression of HSP90 was more than 1.5 times of the reference gene in florescence-leaf, the disease-resistant materials were screened with 95% accuracy. Similarly, when the expression of ATG3 was more than 1.8 times of the reference gene in florescence-flowers, the disease-resistant materials were screened with 80% accuracy. In addition, to combine the expression of HSP90 in florescence-leaf and ATG3 in 5-6 leaf stage leaves can also be used to predict the disease-resistant materials (80% accuracy). The results of this study provids a certain theoretical foundation for screening high oleic acid rapeseed materials with strong disease-resistance in the early stages and promoting the breeding of high oleic acid rapeseed. %U https://www.hnxb.org.cn/EN/10.11869/j.issn.100-8551.2019.05.0894