%0 Journal Article %A JIANG Ming %A ZHANG Zhixian %A ZHANG Huijuan %A GUAN Ming %A CHEN Xiaoshang %A YIN Longfei %A LIU Jie %T Isolation and Expression Analysis of Broccoli Ethylene Response Sensor Gene BoERS %D 2019 %R 10.11869/j.issn.100-8551.2019.01.0016 %J Journal of Nuclear Agricultural Sciences %P 16-23 %V 33 %N 1 %X Ethylene response sensor protein plays an important role in ethylene signal transduction. It acts as a negative regulator which inactivates downstream component CTR1, and triggers a series of reactions succedently. To investigate sequence chavacterristics and expression features of the broccoli ERS gene, our aim is to isolate an ethylene response sensor gene in broccoli and to obtain its expression pattern induced by Sclerotinia sclerotiorum and Plasmodiophora brassicae, thus provide a foundation for its role in disease resistance response. Results indicated that the genomic DNA of BoERS was 1 928 bp in length, containing a 68 bp intron. The full coding sequence was 1 860 bp encoding 619 amino acids, and the deduced protein contained four transmembrane domains, one GAF domain and one HisKA domain. Sequence comparison revealed BoERS showed little difference form ERSs of other Brassica plants, and they were grouped into the same clade, however, larger differences were observed between BoERS and ERSs from Raphanus, Thellungiella and Arabidopsis plants, and they clustered in different clades. Quantitative real-time PCR showed that the expression of BoERS was induced by S. sclerotiorum, and the highest expression level was observed at 36 h with a 3.53 fold increase. Isolation and expression analysis of BoERS laid a basis for gene function identification as well as its application in the future. %U https://www.hnxb.org.cn/EN/10.11869/j.issn.100-8551.2019.01.0016