Journal of Nuclear Agricultural Sciences ›› 2021, Vol. 35 ›› Issue (3): 509-517.DOI: 10.11869/j.issn.100-8551.2021.03.0509

• Induced Mutations for Plant Breeding·Agricultural Biotechnology •     Next Articles

Genetic Diversity Analysis of Iris Hybrid and Mutagenic Breeding Materials Based on SSR Markers

ZHOU Lin, CAI Youming, ZHANG Yongchun*, YANG Liuyan   

  1. Forestry and Pomology Research Institute, Shanghai Academy of Agricultural Sciences/Shanghai Key Laboratory of Protected Horticultural Technology, Shanghai 201403
  • Received:2019-09-29 Online:2021-03-10 Published:2021-01-19


周琳, 蔡友铭, 张永春*, 杨柳燕   

  1. 上海市农业科学院林木果树研究所/上海市设施园艺技术重点实验室, 上海 201403
  • 通讯作者: *张永春,男,研究员,主要从事花卉栽培和种质创新研究。
  • 作者简介:周琳,女,主要从事花卉栽培和遗传育种研究。
  • 基金资助:

Abstract: In order to speed up the breeding process of Louisiana iris, hybridization and 60Co-γ radiation mutagenesis were carried out on five Louisiana iris varieties. Based on the EST-SSR markers of I.brevicaulis and I.fulva, 14 pairs of SSR markers were selected for genetic diversity analysis on Louisiana iris varieties, their hybrid offspring and mutagenic materials. The results showed that there was little genetic difference between the offspring of five Louisiana iris varieties and their maternal plants. However, after irradiation with 60Co-γ, great genetic differences from their parents were observed rays for five Louisiana iris cultivars. The survival rate of tissue culture plants was significantly negatively correlated with the dose of 60Co-γ mutagenesis. And the survival rates of different varieties under treatments of 2.5, 5, 10, 20 and 40 Gy were 20.77%~24.99%, 13.33%~17.89%, 10.11%~15.71%, 3.33~10.14% and 1.11%~2.77% respectively. The phenotypic traits of the mutagenic materials could be observed via tissue culture rapid propagation system. In this study, in vitro culture combined with 60Co-γ radiation mutation breeding was carried out for the first time on Louisiana iris, and 10 pairs of SSR markers were screened for identifying Louisiana iris varieties, hybrid offspring and mutagenic materials were screened. The research provide theoretical basis for Louisiana iris hybrid breeding, radiation mutation breeding and SSR-based germplasm identification.

Key words: iris, simple sequence repeat (SSR) marker, hybrid offspring, irradiation mutagenesis, genetic diversity

摘要: 为加快路易斯安那鸢尾育种的进程,以5个路易斯安那鸢尾品种为试验材料,开展杂交和60Co-γ辐射诱变育种。基于短茎鸢尾和暗黄鸢尾的基于表达序列(EST)开发的微卫星标记(SSR)标记,选择其中14对SSR标记用于路易斯安那鸢尾品种、杂交后代和诱变材料共40份材料的遗传多样性分析。结果表明,5个路易斯安那鸢尾品种的杂交后代均与母本的遗传差异小;而5个路易斯安那鸢尾品种经诱变后获得了与其亲本遗传差异大的诱变材料,5个品种的成活率均随着60Co-γ辐射剂量的成倍增加而显著降低,不同品种在2.5、5、10、20和40 Gy剂量处理下成活率分别为20.77%~24.99%、13.33%~17.89%、10.11%~15.71%、3.33~10.14%和1.11%~2.77%,可通过组培快繁观测其表型性状。本研究利用路易斯安那鸢尾组培苗开展60Co-γ辐射诱变育种,并筛选出10对适用于路易斯安那鸢尾品种、杂交后代和诱变材料的SSR标记,可为路易斯安那鸢尾杂交育种、辐射诱变育种和基于SSR标记的种质鉴定提供理论依据。

关键词: 鸢尾, SSR标记, 杂交后代, 辐射诱变, 遗传多样性