Journal of Nuclear Agricultural Sciences ›› 2019, Vol. 33 ›› Issue (1): 24-30.DOI: 10.11869/j.issn.100-8551.2019.01.0024

• Induced Mutations for Plant Breeding·Agricultural Biotechnology • Previous Articles     Next Articles

Study of Artificial miRNA Regulate Erucic Acid in Brassica napus

WANG Zhiwei1,2, ZHANG Ziyang1, LIN Liting3, ZHANG Jinwen2, LIU Mingjiu1,*, QIAO Yan2   

  1. 1 Collaborative Innovation Center of Modern Biological Breeding Henan Province/Henan Institute of Science and Technology, Xinxiang, Henan 453003;
    2 Agronomy College, Gansu Agricultural University,Lanzhou, Gansu 730070;
    3The International Bilingual Acadermy of AMS of Henan Normal University, Xinxiang, Henan 453003
  • Received:2018-03-09 Revised:2018-08-10 Online:2019-01-10 Published:2018-11-20

Artificial miRNA调控甘蓝型油菜芥酸的研究

王志伟1,2, 张自阳1, 林丽婷3, 张金文2, 刘明久1,*, 乔岩2   

  1. 1 河南科技学院/现代生物育种河南省协同创新中心,河南 新乡 453003;
    2 甘肃农业大学农学院,甘肃 兰州 730070;
    3 河南师大附中双语国际学校,河南 新乡 453003
  • 通讯作者: 刘明久,男,教授,主要从事作物育种及高产理论研究。E-mail: liumingjiu@hist.edu.cn
  • 作者简介:王志伟,男,实验师,主要从事作物生理及分子生物学研究。E-mail:wangzhiwei519@126.com
  • 基金资助:
    河南省基础前沿项目(162300410142)

Abstract: Erucic acid is an important industrial raw material, which is acquied majorly from Brassica napus seeds. To regulate the content of erucic acid in Brassica napus seeds, the specific amiRNA is designed according to the key enzyme gene Bnfad2 in the erucic acid synthesis of Brassica napus L. The expression vector is constructed to transform the high erucic acid Brassica napus cultivar MY15 and low erucic acid Brassica napus cultivar LEA01, and the fatty acid of the transformed plant T0 generation seeds is analyzed. The erucic acid content in transformed erucic canola is up to 5.12% and the lowest is 2.11%; while in the transformed low erucic acid variety, the highest is 0.45%, the lowest is only 0.16%, and the erucic acid content in low acid material is less than 1%; Meanwhile, the content of oleic acid in the transformed seeds is significantly improved, and the lowest is 4.99%, the highest up to 10.71%. The reduction of linoleic acid is quite obvious, and the maximum drop is 17%. The results show that the amiRNA technology can effectively change the composition of fatty acids in the seeds, change their nutritional structure and further increase the added value of rape seeds by influencing the expression of specific genes in process of fatty acid synthesis. In summary, the effect of amiRNA technology is obviously on reduce the expression of FAD2 gene and improve the content of erucic acid in Brassica napus. The technology has great meaning when it's widely used in Brassica napus to adjust the fatty acids composition, change nutrition structure and raise economic value.

Key words: Brassica napus, erucic acid, fatty acids, amiRNA, regulate

摘要: 芥酸是重要的工业原料,主要来源是甘蓝型油菜种子,为调节甘蓝型油菜种子内芥酸含量,针对甘蓝型油菜芥酸合成过程中的关键酶基因Bnfad2设计特异性amiRNA,并构建种子特异表达载体,转化高芥酸甘蓝型油菜品种MY15和低芥酸甘蓝型油菜品种LEA01,并对转化植株T0种子脂肪酸进行分析。结果表明,转化的高芥酸甘蓝型油菜芥酸的增加最高达到5.12%,最低2.11%;而转化的低芥酸品种,最高仅为0.45%,最低只有0.16%,而且所有转化低芥酸材料的芥酸含量都小于1%;同时转化种子内油酸的含量得到了明显的提高,最低为4.99%,最高达到了10.71%,亚油酸降低的幅度十分明显,最大降幅达到17%。表明amiRNA技术可以通过影响脂肪酸合成过程中特定基因的表达,有效改变种子内脂肪酸的组成,从而改变其营养结构,提高营养价值,进一步提高油菜种子的附加值。本研究为运用amiRNA技术调节油菜脂肪酸组成和改变其营养结构提供了一定理论依据。

关键词: 甘蓝型油菜, 芥酸, 脂肪酸, amiRNA, 调控