To enrich the resources of seed specific promoters, we PCR-amplified the promoter AHSSP1 of seed storage protein PSC32 from peanut genome. The expression patterns of PSC32 gene was detected by semi-quantitative PCR, cis-acting elements in AHSSP1 were analyzed using online NewPLACE. GUS gene driven by AHSSP1was transformed into Arabidopsis. The function of AHSSP1 was characterized through GUS histochemical staining. Results showed that AHSSP1 ahd 957 bp in length, with 3 RY REPEAT, which were commonly dispersed in seed specific promoters. PSC32 gene was found to be expressed specifically in seed, but not expressed in root, stem, leaf, flower, peg, and pod shell of mature seed in pod maturing stage based on semi-quantitative PCR analysis. Histochemical staining of Gus activity showed that mature seeds of transgenic Arabidopsis, and the cotyledons, hypocotyl and young root of germinating seeds were all stained blue. Cotyledon and hypocotyl still exhibited GUS staining activity after growing true leaves, while the roots and true leaves did not display GUS staining activity. The leaves of adult transgenic Arabidopsis could not be stained blue, while non-transformed Arabidopsis could not be stained blue throughout the growth period. It indicated that AHSSP1 was a seed specific promoter. This study enriched the resources of peanut seed specific promoters, and would play important application in improving peanut kernel quality or using peanut seed “bioreactor” research.