To study the inheritance pattern of a new male sterile mutant in maize. The sibling population of maize K305 male sterile mutant induced by 60Co-γ irradiation was planted in different locations, years and seasons, the male sterility was identified by using interior microscopic examination of pollen and the field investigation method. The nucleus effects were analyzed by the sister crosses, test crosses, and their selfing and backcross progenies with the sterile plants as females. The cytoplasm effects were investigated through analyzing the reciprocal F1 and F2 with normal inbred lines as females, and the fertility fully recovered test crosses and fertile sibling as males, respectively. The results showed that the male sterile plant exhibited closed glumes, empty anther, complete and stable sterility. The segregation ratio of fertile to sterile plants was 1:1 in the sibling and backcross populations, while 3:1 in the fertile selfing population, and the heredity of the male sterility was not associated with the varied cytoplasm. The demonstrated that the mutant, belonged a pollen-less type, was a genic male sterility controlled by a single recessive gene, which laid the foundation for further utilization.
The green globular bodies (GGB) of Asplenium nidus, Neocheiropteris palmatopedata(Baker)Christ and Pteris cretica were used for 60Co-γ rays irradiation. In the 1/2 MS modified medium supplemented with 0.2 mg·L-1 IBA, GGB were irradiated at the dose rate 1 Gy·min-1 of 60Co γ-rays. The results showed that the shooting rate was from 98% to 25% when GGB irradiated with 0 and 60 Gy in Asplenium nidus. The shooting rate was from 100% to 38% and 26% when GGB irradiated with 0 or 80 Gy in Neocheiropteris palmatopedata(Baker)Christ and Pteris cretica respectively. The suitable dosage of 60Co-γ rays were 60 to 80 Gy for shooting from GGB in pteridophyte. The result of SSR markers test demonstrated that the specific SSR markers were associated with the mutants, among which, SSR-23 and SSR-76 were suitable for identification for screening mutants in Asplenium nidus. In summary, the mutation technique of 60Co-γ rays irradiation has been established in GGB of pteridophyte.
To investigate the differences between electron beam irradiation and gamma irradiation, chilled pork was irradiated through two irradiation treatments and study the lipid oxidation, nutritional, physicochemical and sensory qualities indicating the quality of chilled pork. The results showed that the TBARS、carbonyl values and dienes values of irradiated chilled pork increased significantly, and these indexes were higher in gamma irradiation groups. The a*, a*/b*, cooking loss and shear force of irradiated samples were significantly higher than control group and the odor in irradiated samples was better comparing with control samples. The content of fat、protein and moisture have no significantly change. In conclusion, irradiation treatment accelerate the oxidation of chilled pork, but the electron beam irradiation had slighter oxidation effect compared with isodose gamma irradiation and the nutritional、physicochemical and sensory qualities were better than gamma ray irradiation, thus the electron beam irradiation had better effect in chilled pork preservation. The research was helpful for guiding the application of electron beam irradiation in chilled pork preservation.