Most Download

Published in last 1 year | In last 2 years| In last 3 years| All| Most Downloaded in Recent Month | Most Downloaded in Recent Year|

Most Downloaded in Recent Year
Please wait a minute...
For Selected: Toggle Thumbnails
Effects of Nitrogen Application Amount on Formation and Yield of Potato Tuber Starch in Ningxia Arid Region
LIU Qiangjuan, KANG Jianhong, WU Jiarui, SUN Jianbo, MA Xueying, WANG Xingqiang, JIAN Tiancai
2021, 35 (5): 1196-1208. DOI: 10.11869/j.issn.100-8551.2021.05.1196
Abstract92)   HTML1)    PDF (1930KB)(1022)      
For exploring the effect mechanism of different nitrogen application amounts impact on potato tuber starch formation and yield in Ningxia arid areas, field trials were carried out in the mountainous areas of southern Ningxia in 2017(normal year) and 2019(wet year) respectively. The potato cultivar Qingshu No. 9 was used as the material and a single factor random Block experiment was designed, with no nitrogen fertilizer (N0) as the control, to study the effects of pure nitrogen application rate of 75 (N1), 150 (N2), 225 kg·hm -2 (N3) on potato starch content, key starch forming enzymes and yield. The results showed that with the increase of nitrogen application amount, the total starch and amylopectin content increased first and then decreased, with N2 being the highest, significantly increasing by 21.23, 26.42 percentage point (2017) and 19.74、24.93 percentage point(2019); while the amylose content increased with the increase of nitrogen application amount, reaching the highest at N3, which was 1.59 percentage point (2017) and 1.78 percentage point (2019) higher than N0. Both adenosine diphosphate glucose pyrophosphorylase (AGPP) and starch branching enzyme (SBE) had the highest N2, which increased by 57.38%, 36.83% (2017) and 67.19%, 29.01% (2019) compared with N0, respectively; while soluble starch synthase (SSS) and bound starch synthase (GBSS) were the highest with N3, which increased by 33.90%, 49.51% (2017) and 46.02%, 51.00% (2019) respectively compared with N0. Correlation and general analysis show that starch content and key enzymes in starch synthesis have a positive correlation, and AGPP, SSS, and GBSS have a greater direct contribution to the process of starch synthesis; Compared with N0, the potato output of N1~N3 increased by 10.72%~21.02% and 8.40%~16.30% in 2017 and 2019, respectively. The yield of N2 is the best; the best nitrogen application rate to achieve the highest yield is 150-184 (2017), 150-196 kg·hm -2 (2019). Therefore, it is suggested that the nitrogen application amount of 150-200 kg·hm -2 for potato in arid areas of Ningxia can effectively increase the activity of key enzymes in starch formation, accelerate starch formation and accumulation, and promote the increase of potato production, thereby increasing the economic benefits of local people.
Identification of MIR319 Family Members and Their Target Genes in Response to Cucumber Green Mottle Mosaic Virus Infection in Watermelon
SUN Yuyan, ZHANG Huiqing, FAN Min, HE Yanjun, GUO Ping'an
2021, 35 (5): 1048-1059. DOI: 10.11869/j.issn.100-8551.2021.05.1048
Abstract125)   HTML1)    PDF (16143KB)(1206)      
In order to reveal the possible roles of MIR319 family (miR319, miR319a and miR319a-3p) under the CGMMV stress, mature sequences of MIR319 were blasted against the watermelon genome to obtain the precursor gene. MEGA was used to analyze the evolutional relationship of precursor genes for MIR319. PlantCARE was used to analyze the cis-acting regulatory elements of the precursor gene promoter. Degradome sequencing was used to identify target genes of MIR319, and transcriptome sequencing and qRT-PCR were used to obtain the expression of target genes. The common precursor gene of MIR319, Pre-MIR319 was obtained, which was 170 bp in length, able to form the stem-loop structure. Sequence alignment showed that mature sequences of MIR319 were highly conserved at the 2~14 bases of 5'-terminal. Phylogenetic analysis of watermelon Pre-MIR319 and 116 miR319 precursor sequences from 35 species divided these precursor sequences into four branches. Watermelon Pre-MIR319 was closest to potato precursor gene miR319 a (MI0025952). The promoter of Pre-MIR319 contain several cis-acting regulatory elements, such as light responsive element, gibberellin responsive element, ethylene responsive element, methyl jasmonate response element, MYB, MYC and etc. Five target genes of MIR319 family, Cla019567, Cla013523, Cla023342, Cla002428 and Cla013668, were predicted by degradome sequencing results. Among which, Cla019567, Cla013523, Cla023342 and Cla002428 are annotated as TCP transcription factor and Cla013668 is annotated as MYB transcription factor. The cleavage sites were located at the 10 th of MIR319 at the 5'-terminal end. The amino acids number, molecular weight and theoretical isoelectric point of target genes were 319~554 aa, 34.94~61.21 kDa and 5.29~7.80, respectively. These proteins do not contain transmembrane domains and was located in the nucleus/cytoplasm. Expression profiles of target genes using transcriptome and qRT-PCR analysis revealed that miR319a negatively regulated its target gene Cla013523 ( TCP). These results clarified the role of MIR319 family members in CGMMV stress response and revealed the regulation of MIR319 on their target genes.
Effect of 60Co-γ Irradiation on Capsule of Epidendium secundum
ZHOU Yaqian, YAO Na, WEI Li, LI Lubin, LIU Lei
2017, 31 (9): 1693-1699. DOI: 10.11869/j.issn.100-8551.2017.09.1693
Abstract197)   HTML1)    PDF (1220KB)(884)      
To investigate the effect of the 60Co-γ ray treatment on the Epidendrum secundum capsule, different radiation doses of 60Co-γ ray (0 to 200 Gy) were used in this study, and the effect of radiation on the seeds germination and the seedling growth were investigated. The results showed that 20 Gy radiation dose can promote the germination rate compared with the control group, and the germination time was shortened. Under the radiation dose of 200Gy, the seeds could not germinate. The semi-lethal dose (LD 50) was 78.08 Gy. The biological statistics analysis indicated the radiation dose had larger impact on plant height and leaf length, and had little effect on blade quantity and less influence on the leaf width. These results will lay the theory basis for radiation breeding of Epidendium secundum.
Transcriptome Analysis and Gene Function Annotation of Early Developmental Broccoli Microspores Based on High-throughput Sequencing Technology
ZHANG Zhenchao,YAO Yuemei,MAO Zhongliang,SUN Guosheng,QIN Wenbin,DAI Zhongliang
2018, 32 (5): 848-855. DOI: 10.11869/j.issn.100-8551.2018.05.0848
Abstract173)   HTML1)    PDF (9287KB)(933)      
In order to investigate the molecular biological mechanisms of broccoli microspore embryogenesis, the Illumina HiSeq TM 2000 platform was utilized to analyze and annotate transcriptomes of the microspores, cultured at 32.5℃for 17 h and 24 h and at 25℃ for 0, 1, 6 d in this research. The results showed that a total of 174324 Unigenes were produced, among which 144 194 were annotated in GO、COG、KEGG、NR、Swissprot and Pfam database. The analysis of gene ontology function enrichment revealed that some important biological processes related to microspore embryogenesis, such as cell part, cell and organelle, binding and catalytic activity, metabolic process, cellular process and single-organism process, etc, got significantly enriched. The frequencies of R、L、K、T and O were much higher than others in COG function classification. The results of KEGG pathways enrichment showed that differentially expressed genes produced from microspores after heat shock tended to cluster into the pathway of protein processing in endoplasmic reticulum, the pathways of plant-pathogen interaction, plant hormone signal transduction and spiceosome, etc. The results laid a good foundation for further study on the physiological and biochemical and molecular mechanisms of broccoli microspore embryogenesis.
Identification and Analysis of Heat Tolerance Identification and Analysis for Brassica campestris ssp. chinensis
ZHANG Jing-yun, ZHAO Xiao-dong, WAN Xin-jian, XIONG De-tao, HU Xin-long, MIAO Nan-sheng
2014, 28 (1): 146-153. DOI: 10.11869/j.issn.100-8551.2014.01.0146
Abstract309)      PDF (7855KB)(536)      
Sixty-eight varieties of Brassica campestris ssp. chinensis were tested and screened for heat tolerance based on phenotype evaluation in field, and varieties with various heat tolerances were measured for their malondialdehyde content, relative leaf membrane permeability and proline content. The results showed the five tolerant varieties and five sensitive varieties selected were increased in malondialdehyde content, relative leaf membrane permeability and proline content under heat stress as time proceeded, but significant differences were found between the two groups of varieties. Therefore, malondialdehyde content, membrane permeability and proline content could be used as physiological indices for identification of heat tolerance in Brassica campestris ssp. chinensis.
Comparative Study on Sugar Metabolism in Keitt Mango Fruit Between Bagging With Yellow and White Bags During Postharvest
WU Lina, ZHU Yuyan, HUAN Chen, XU Qihang, LI Sheng'e, ZHENG Xiaolin
2021, 35 (5): 1121-1128. DOI: 10.11869/j.issn.100-8551.2021.05.1121
Abstract100)   HTML1)    PDF (3533KB)(461)      
In order to explore the effects of different colored bagging on postharvest soft nose and storage characteristics, the incidence of soft nose and sugar metabolism in mango fruit Mangifera indica Keitt pre-harvest bagged with yellow or white bag were investigated during storage. The results showed that the bagging with yellow bag significantly reduced the incidence of soft nose disease in mango fruit during storage for 10 d compared to bagging with white bags. In addition, the bagging with yellow bags significantly decreased the activities of amylase, acid invertase (AI), neutral invertase (NI), sucrose phosphate synthase (SPS) and sucrose synthase (SS) in flesh of the mango fruit, which resulted in slowing the rates of decrease in starch content and accumulation of soluble sugar content, and in turn to be contributed to delaying the softening rate and improving the fruit storability of mango fruit during storage. It was suggested that the bagging treatment affected the sugar metabolism would be associated with the development of soft nose disease in Keitt mango fruit during postharvest. Our present work might provide references for selecting reasonable pre-harvest bagging materials of mango fruit in practice.
Cloning and Deletion Analysis of NtMTPC4 Promoter From Nicotiana tabacum
LIU Jikai, ZHANG Lin, GAO Yongfeng, WU Chanjuan, TANG Yunlai
2017, 31 (7): 1282-1289. DOI: 10.11869/j.issn.100-8551.2017.07.1282
Abstract159)   HTML0)    PDF (6607KB)(575)      
Tissue-specific promoters are important tools in genetic engineering and practical application. In order to study the expression characteristics of NtMTPC4 promoter from Nicotiana tabacum, the relative expression of NtMTPC4 was determined by quantitative real-time PCR (qRT-PCR). A 2 287 bp length promoter sequence of NtMTPC4 gene was cloned by PCR method. Based on the distribution of the regulatory elements, three truncations of NtMTPC4-P were obtained. The full length NtMTPC4-P and the truncations were then inserted into pBI121K respectively to replace the CaMV35S promoter. All the recombinant plasmids were introduced into Arabidopsis by Agrobacterium-tumefaciens mediated floral dipping method. qRT-PCR analysis indicated that NtMTPC4 gene was highly expressed in flowers. Sequence analysis showed that NtMTPC4-P contained basic cis-elements, such as CAAT-box, TATA-box and tissue specific regulation elements, GTGANTG10 and POLLEN1LELAT52. GUS histochemical assay showed that the expression of the GUS in all of the transgenic plants was detected particularly in pollens. These results provide a new regulatory element for the expression of target gene specifically in pollen by genetic engineering.
Nitrate Uptake, Transport and Signaling Regulation Pathways
LI Chenyang, KONG Xiangqiang, DONG Hezhong
2020, 34 (5): 982-993. DOI: 10.11869/j.issn.100-8551.2020.05.0982
Abstract233)   HTML2)    PDF (3084KB)(1046)      

Nitrate is the major forms of nitrogen that plants absorb from the soil, and its uptake and utilization is a highly coordinated and complex process. For the sake of survive in various environmental conditions, plants have evolved suitable nitrate uptake and utilization mechanisms to adapt to a wide range of environmental conditions. There are various types of nitrate receptors in plant roots, which can sense different concentrations of external nitrate. The low- or high-affinity nitrate uptake system was activated to absorb nitrate from the environment according to the levels of external nitrate. Once nitrate is taken up into root cells, most of it is transported to shoots for assimilation, and synthesizes macromolecular substances to optimize plant growth. When the nitrate supply is too much for use immediately, the plant can store the excess nitrate into vacuoles and mediate it efflux from vacuoles to the cytosol for assimilation when needed. During plant growth and development, nitrate in the old and mature leaves can be redistributed into developing tissues to promote their growth. Many genes related to nitrate absorption, transportation, storage, assimilation and signaling regulation are activated orderly and work coordinately to absorb and utilize nitrate efficiently. This review summarizes the NRT1 and NRT2 nitrate uptake-related genes and their functions, as well as related transcription factors involved in primary nitrate response and small signal peptides in nitrate signaling transduction and exchange among different tissues. In order to further understand the mechanisms of plant uptake and utilize nitrate, and therefore provides new ideas in increasing nitrogen-use efficiency of crop by breeding and cultivation techniques.

Review on Chemical Structures and Biological Activities of Spermidine Derivatives in Plants
ZHAO Shuo, XING Cencan, WANG Yan, SUO Ran, WANG Fengzhong
2018, 32 (1): 123-130. DOI: 10.11869/j.issn.100-8551.2018.01.0123
Abstract155)   HTML0)    PDF (1167KB)(678)      
Spermidine derivatives, which widely existed in the pollen, leaf and fruit in the plants, are a kind of natural compounds which the hydrogen atom of spermidine is replaced by other functional group. The structural identification, purification, especially the biological activities of spermidine derivatives are still worthy to explore. This review summarizes the chemical structures, sources, purification and biological activities of spermidine derivatives reported in the past 30 years, which may possibly build a foundation for future research of spermidine derivatives with special functions.
Effect of Different Irrigation Regimes on Rice Yield and Water Use Efficiency Under Straw Returning to Field
ZHAO Hongliang,WANG Qi,SUN Yu,ZENG Xiannan,ZHANG Xiaoming,WANG Ping,WANG Manli,FENG Yanjiang
2018, 32 (5): 959-969. DOI: 10.11869/j.issn.100-8551.2018.05.0959
Abstract179)   HTML0)    PDF (1445KB)(612)      

In order to explore the application effect of straw returning to field combined with water-saving irrigation technology, the effect of different irrigation regimes on rice growth and development, yield and its components, and water use efficiency was studied under the condition of straw returning to full field. The results showed that as for rice growth and development, to some extent, straw returning to field combined with water-saving irrigation technology could increase tillers number in the early middle period, decrease plant height at maturity, reduced the panicle formation percentage with the decrease of irrigation amount. In the early middle period of rice growth and development, proper reduction of water supply is beneficial to the accumulation of dry matter in leaves and stems and sheath, the dry matter output rate and transfer rate of leaves were the largest under the treatment of control irrigationⅠ, arriving at 14.19% and 4.15%, and the dry matter output rate and transfer rate of stems and sheath were the largest under the treatment of intermittent irrigation,arriving at 28.69% and 17.39%, respectively. As for yield and its components, the yield order of the different irrigation regimes was intermittent irrigation>flooding irrigation>control irrigationⅠ>control irrigationⅡ. As for water use efficiency, irrigation water use efficiency was the largest under the treatment of control irrigationⅡ with 3.55 kg?m-3, and natural rainfall water use efficiency was the largest under the treatment of control irrigationⅡ,arriving at 1.10 kg?m-3. Total water use efficiency was the largest under the treatment of control irrigationⅠ, arriving at 0.75 kg?m-3. The results concluded that, under the condition of straw returning to field and ensuring yield, the best irrigation regimes were intermittent irrigation and control irrigation I.The results could provide theoretical basis for water management under straw returning to field in Northeast China, increase water use efficiency and improve rice production sustainable development.

Research Progress on Promoting Growth and Drought Resistance of Wheat by Plant Growth Promoting Rhizobacteria
WEN Hongwei, YANG Bin, WANG Dongsheng
2021, 35 (9): 2194-2203. DOI: 10.11869/j.issn.100-8551.2021.09.2194
Abstract141)   HTML7)    PDF (77943KB)(325)      

Drought stress is the most important abiotic stress factor affecting the growth and development and yield formation of wheat. With the adverse effects of excessive use of chemical fertilizer and global climate change, the harm of drought stress in the growth of wheat is becoming more and more serious. Plant growth promoting rhizobacteria (PGPR) can not only help wheat improve its nutrient utilization efficiency and promote its growth, but also help wheat to resist drought stress through its own action or various metabolites. In this paper, the mechanism of PGPR promoting growth was summarized, and the research progress of PGPR in improving wheat drought resistance was summarized from two aspects of physiological and molecular mechanism, which laid a theoretical foundation for the application research of using PGPR to improve wheat growth promotion and drought resistance.

Expression and Function of Cauliflower BobERF17, A Member of the AP2/ERF Transcription Factor Family, In Response To Abiotic Stresses
LI Hui, YANG Yaling, LI Cong, LI Lihong, HAN Zhanpin, WANG Chunguo
2021, 35 (8): 1794-1801. DOI: 10.11869/j.issn.100-8551.2021.08.1794
Abstract118)   HTML2)    PDF (7166KB)(318)      
AP2/ERF is a plant-specific transcription factor superfamily, among which the members of ERF family have been identified to play important roles in abiotic stress responses in plants. To further understand the role of ERFs in cauliflower, in the present study, BobERF17, a member of the ERF family in cauliflower, was cloned, and the transcriptional expression and function of BobERF17 were further explored. Sequence analysis showed that BobERF17 coding region is 576 bp in length and encodes a protein consisting 191 amino acids with unknown function. Cluster analysis showed that BobERF17 had a high similarity to the ERF17 sequences in dicotyledons, especially Brassica plants, but differed greatly from the ERF17 transcription factors in monocotyledons. Analysis of expression profiles under different stress conditions confirmed that BobERF17 displayed significantly up-regulated expression characteristics under drought and high temperature stresses, but was not sensitive to salt and low temperature stresses. Subsequently, the BobERF17 overexpression vector was successfully constructed and transformed into Arabidopsis. Overexpressed BobERF17 Arabidopsis homozygous lines were obtained. Functional analysis showed that the overexpression of BobERF17 in Arabidopsis did not affect the growth and development of the transgenic lines, but could significantly increase their tolerance to drought and high temperature stresses. The results confirmed that BobERF17 may play an important role in positive response to drought and high temperature stresses in cauliflower. These findings provided new insight into the function and regulation of BobERF17 in cauliflower.
Antifungal Activity and Possible Mechanism of Clove Essential Oil on Dominant Pathogens of Postharvest Sweet Cherries
WANG Dan, ZHANG Jing, JIA Xiaoman, ZHAI Hao
2020, 34 (6): 1221-1229. DOI: 10.11869/j.issn.100-8551.2020.06.1221
Abstract271)   HTML2)    PDF (4580KB)(645)      
The paper aimed to reveal the antifungal activity and possible mechanism of plant essential oil on dominant pathogens of postharvest sweet cherries. Hongdeng, the main varietiy in Taian, Shandong Province, was chosen as the raw material, traditional morphological identification method combined ITS analysis was performed to separate dominant pathogens from rotten sweet cherries, and antifungal activity of clove essential oil on three pathogenic fungal strains was assessed by in vitro fumigation and contact. The results showed that these strains were identified as Botrytis cinerea, Alternaria alternate and Colletotrichum gloeosporioides. Clove oil at certain concentrations exhibited obvious antifungal effects by inhibiting mycelial growth and spore germination of the three strains, and fumigation was better than contact. For B. cinerea, A. alternate and C. gloeosporioides, the minimal inhibitory concentrations (MIC) of clove oil in air phase were 120, 100 and 100 μL·L -1 in vitro testing, respectively. Moreover, the possible mechanisms of fumgitoxic action were related to the hydrophobicity of essential oils which enabled them to disrupt membrane structure of microorganism, and lead to loss of cell contents. In conclusion, clove essential oil was proved to be a promising candidate as effective antifungal agent for postharvest decay of sweet cherries.
Difference of Curing Characteristics of Upper Leaves With Different Maturity in Different Flue-cured Tobacco Varieties
WU Shengjiang, MO Jingjing, LOU Yuanfei, TU Yonggao, ZHAO Huina, ZHAN Jun, WEI Kesu, ZHAO Degang
2020, 34 (6): 1337-1349. DOI: 10.11869/j.issn.100-8551.2020.06.1337
Abstract224)   HTML4)    PDF (1275KB)(624)      
In order to investigate curing characteristics of upper tobacco leaves, the difference of water loss rate, yellowing and browning characteristics, chlorophyll relative content (SPAD), color values, polyphenol oxidase (PPO) activity and chemical composition content of the different maturity leaves of K326, Bi’na1, Zunyan6, Guiyan1, Guiyan4 and Honghuadajinyuan (Hongda) were studied during curing or during drawer testing. The results showed that Bi’na1 had smaller water loss rates while Hongda and Guiyan1 had larger water loss rates; Bi’na1 and K326 had shorter yellowing time while Hongda had a longer yellowing time during curing and drawer testing. The average PPO activity of Bi’na1 was less than 0.4 U and that of Guiyan1 was larger than 0.5 U during curing. As for chlorophyll relative content, Hongda had larger SPAD values, then were Zunyan6 and Guiyan1, and the last were Bi’na1, Guiyan4 and K326 during drawer testing. Hongda and Zunyan6 showed a relatively poor color values, and Guiyan1 showed a more serious browning. However, Bi’na1 had a lighter browning during drawer testing. Moreover, flue-cured tobacco leaves of Bi’na1, Guiyan4 and Zunyan6 had a relatively suitable chemical composition content. Following progressive improvement of tobacco leaves maturity levels, the yellowing time of fresh tobacco leaves was shortened and the activity of PPO increased during curing, the total content plant alkaloid and nitrogen decreased, and the content of water-soluble total sugar and reducing sugar increased. In addition, the yellowing time and browning time of tobacco leaves were shortened, the SPAD value was reduced, and the color showed better before the leaf became browning with the improvement of tobacco leaf maturity levels during drawer testing. Variety and maturity not only affected the leaf phenotype, but also had a significant impact on its intrinsic physiology. The curing characteristics of different flue-cured tobacco varieties were not only closely related to the chemical composition, but also the genotypes. As for drawer testing, SPAD value could be used as a phenotypic indicator for judging the easy curing portential, and the color parameters could be used as a phenotypic indicator for judging the easy and endurable curing portential. Taken together, K326, Bi’na1, Guiyan1 and Guiyan4 had a better easy curing portential, then was Zunyan6, and Hongda showed the worst performance. In addition, Bi’na1 had a better endurable curing portential, then were K326, Guiyan4, Hongda and Zunyan6, and Guiyan1 showed the worst performance. According to the difference of curing characteristics of different flue-cured tobacco varieties, we have put forward some specific measures for different tobacco varieties curing, which lay the theoretical foundation for different flue-cured tobacco varieties curing and the improvement of upper tobacco quality.
CHENG Long-jun GUO De-ping ZHU Zhu-jun SUN Yun-zi(Department of Horticulture, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou,Zhejiang, 310029)
2004, 18 (06): 457-461. DOI: 10.11869/hnxb.2004.06.0457
Abstract1379)      PDF (297KB)(1551)      

The contents of carbohydrates (total sugar, reducing sugar, sucrose, starch, lignin and cellulose), protein and activities of some enzymes (superoxide dismutase, catalase, peroxidase, and phenylalanine ammonia-lyase) in swollen stem, which is used as a vegetable in Chinese kitchen, of gausun (Zizania latifolia L.) were measured during stem swelling. The results showed that the stem weight increased rapidly at early phase, then both weight and volume of the swollen stem rose at later phase. The contents of sugar and reducing sugar increased during the early stage, then decreased. The sucrose content also increased during stem swelling, then kept a stable level. The contents of lignin and cellulose declined with stem swelling, they started to rise after reached the lowest level. An increase in protein was observed at early swelling stage, then decreased, while starch level rose during stem swelling. The activities of catalase and superoxide dismutase declined continuously during stem swelling. However, peroxidase activity increased slowly at earlier swelling stage, and rapid rise occurred afterwards. The possible correlation between these biochemical changes and the formation of the swollen stem were discussed.

Multi-index Fuzzy Evaluation of the Effect of Different Sowing and Transplanting Date on Flue-cured Tobacco Yield and Quality
LI Diqin, GONG Zhanwu, CHEN Yifan, LIU Guanghui, YAO Xuemei, XIAO Bo, LI Yuhui, ZHANG Guangli, ZHANG Zhen
2017, 31 (11): 2258-2264. DOI: 10.11869/j.issn.100-8551.2017.11.2258
Abstract144)   HTML0)    PDF (1289KB)(621)      
For exploring the suitable sowing and transplanting time of the flue-cured tobacco in Longhui flue-cured tobacco planted areas, xiangyan3 as a material, the evaluation of the flue-cured tobacco appearance quality, the economic character index(ECI) and chemical components usability index(CCUI) of the principal grade flue-cured tobacco leaves with different seeding and transplanting time was made by a fuzzy evaluating and weighted method in 2014 and 2015. The result shows as follow: the score of appearance quality index evaluation, the economic character index(ECI) and chemical components usability index(CCUI) of the principal grade flue-cured tobacco leaves, and the weighted value of the three indexes are the best with the treatments of “seeding on 30 th Dec. and transplanting on 4 th Apr.”. With the delay of the sowing and transplanting time, variation of score of appearance quality index evaluation, ECI and CCUI of the principal grade flue-cured tobacco leaves follows the law of “low-high-low”. Based on the above mentioned analysis, the suitable seeding time in the Longhui areas is late Dec. to early Jan. and transplanting from the end of Mar. to early Apr.”, which brings the benefit of increased yield and quality. This study provides the theoretical basis for setting suitable flue-cured tobacco seeding and transplanting time in Longhui flue-cured tobacco planting areas.
Marker Encryption and Candidate Gene Prediction of Sugar Content-Related QTL in Pumpkin
WANG Manman, QU Shuping, HUANG Hexun, XUE Shudan, WU Tingquan, LI Junxing, DAI Zuyun, ZHONG Yujuan
2020, 34 (11): 2407-2415. DOI: 10.11869/j.issn.100-8551.2020.11.2407
Abstract183)   HTML2)    PDF (5884KB)(520)      
In order to understand the inheritance of sugar-related traits in pumpkin fruit, two high generation of inbred lines with significant difference in sugar content and composition, the guangdong local high line (CMO-97) and Thailand line (CMO - E), were used as the parents to build F 2 segregation population in this study. Based on the high-throughput sequencing and parental genome resequencing results, molecular markers linked to the sugar-related trait of the pumpkin were developed. The markers located in the original location mapping region of sucrose and glucose ratio (qs/g19-a) on the genetic map was encrypted. The key genes controlling sweetness of pumpkin were predicted and molecular markers closely linked to fruit sugar content were screened. The results showed that 9 pairs of InDel markers and 6 pairs of KASP markers were polymorphic among the 50 pairs of InDel markers and 12 pairs of KASP markers. The encrypted linkage group 19 contained 6 KASP markers, 9 InDel markers and 112 SNP markers. The qs/g19-a localization region was narrowed from 968.7 to 356.3 kb. Compared with the reference genome information, we found that there were 56 genes in the narrowed localization region, among which there were 4 candidate genes related to sugar content. The results of this study provide reference and molecular marker resources for breeding studies on pumpkin sugar content.
Research Advances in Plant Stress Resistance Regulated by Signal Molecule Hydrogen Sulfide
TIAN Yun, JIANG Jinglong, LI Li, YU Miao, REN Xuming
2017, 31 (11): 2279-2287. DOI: 10.11869/j.issn.100-8551.2017.11.2279
Abstract169)   HTML1)    PDF (4316KB)(843)      
Hydrogen sulfide(H 2S) is a novel type of active endogenous signal molecule following carbon monoxide(CO) and nitric oxide(NO), which plays an important role in plant seed germination, photosynthesis stomatal movement, lateral root formation and delaying plant senescence. Effects of H 2S on plant resistance to biological or abiological stress have been reported in many papers. The objective of this study was to review the molecular mechanism of H 2S synthetic metabolic pathway and the research advances in H 2S alleviating various plant stressses, including heavy metals, high salt, low oxygen, drought, high temperature and low temperature and so on. The molecular mechanism of interaction between H 2S and other signal molecules was also discussed, which is intended to provide a reference for further study of the mechanism of H 2S.
Influence of Different Mulching Materials on Soil Microbe and Grape Growth in Rain-Shelter Vineyard
LUO Ling, ZHONG Qi, WANG Jin, PAN Hongbing, LIU Wei
2021, 35 (2): 471-480. DOI: 10.11869/j.issn.100-8551.2021.02.0471
Abstract110)   HTML0)    PDF (1630KB)(403)      
To explore the mulching effect of different mulching materials in rain-shelter vineyards, a field experiment was designed, and non-mulching (CK) as control, and four mulching materials of Straw Mulch (SM), White Plastic Film Mulch (WM), Reflective Plastic Film Mulch (RM), Ground Cloth Mulch (CM) were treated continuously from November 2017 to October 2018. Taking the summer black grape as the sample, the effects of different mulching materials on soil microbe and grape growth in rain-shelter vineyards were studied. The results indicated that, compared with the control check (CK), by CM, WM and RM treatment, the number of fungi and bacteria in 0~40 cm soil layer could be increased and soil actinomycetes could be reduced; soil microbial biomass carbon and soil microbial quotient under the four mulching materials were all higher than CK, while microbial metabolic quotient was lower than the control; soil organic carbon under CM, WM and RM treatment showed a decreasing trend and under SM treatment was on the contrary; SM and CM treatment could increase soil respiration intensity, and WM and RM treatment were on the contrary; the four mulching materials could increase the grape root activity, promote the growth of grape leaves and new shoots, and improve the internal and external quality of grape. The quantity and activity of soil microorganism under SM treatment were the strongest, whose total microbial biomass, soil microbial biomass carbon, soil microbial quotient and soil respiration intensity significantly increased by 20.27%, 51.43%, 40.38%, and 13.52%, respectively, while soil microbial metabolic quotient significantly decreased by 22.51% compared with the control; Grape fruit quality was better under SM and RM treatment, but no significant difference was found between them. Compared with the control, the soluble solids of SM and RM treatment significantly increased by 14.25%, and 17.74%, respectively. The experiment results provided theoretical and practical references for the selection of mulching materials in rain-shelter vineyards.
Traits Analysis of Offsprings Mutated by 60Co-γ Radiation in Iris hollandica
LIN Bing, FAN Ronghui, CHEN Yuhua, FANG Nengyan, YE Xiuxian, ZHONG Huaiqin, HUANG Minling
2021, 35 (10): 2205-2213. DOI: 10.11869/j.issn.100-8551.2021.10.2205
Abstract178)   HTML34)    PDF (8438KB)(276)      

In order to explore distinguishing main characters of mutant obtained by 60Co-γ radiation in Iris hollandica Zhanchi, botanical agronomic traits of 4 stable mutants (5-7CBS, 7-10SLZ, 7-10QLS, 10ZLL) and their wild-type Zhanchi were evaluated, the similarities and differences of the main ornamental traits (flowers) were analyzed and compared according to test guidelines of Bulbous Irises. It was found that mutants and wild type were basically consistent in plant morphology, flowering period and growth period. Among the 40 traits investigated, there were significant differences in the characteristics of five pseudo-qualitative characteristics between mutants and wild type, such as groud color of upper side of blade in outer tepal, color of inner tepal, color of upper side in bridge, color of upper side in crest, and groud color of upper side of claw in outer tepal. Another 35 character were highly similar in corresponding phenotype and values. The results showed that flower color of Iris hollandica has great genetic improvement potetiality with radiation breeding. It is speculated that flower color variation may be an important indicator for distinguishing mutants from wild type. According to the expression state of flower color, variation of flower color can be divided into four types: strengthening type, wild type, weakening type and full variant type. This study can provide basic data and reference for breeding new varieties in Iris hollandica.

Announcement More

  • 2021-10-19

  • 2020-12-31

  • 2020-11-03

  • 2020-01-10

  • 2020-01-10

  • 2019-01-25

  • 2019-01-24

  • 2019-01-04

  • 2017-12-25

  • 2017-05-19

  • 2017-02-27

  • 2017-01-18

  • 2016-01-20

  • 2016-01-06

  • 2015-11-27
Editorial Information
Most Read More
Links More