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Identification and Analysis of Heat Tolerance Identification and Analysis for Brassica campestris ssp. chinensis
ZHANG Jing-yun, ZHAO Xiao-dong, WAN Xin-jian, XIONG De-tao, HU Xin-long, MIAO Nan-sheng
2014, 28 (1): 146-153. DOI: 10.11869/j.issn.100-8551.2014.01.0146
Abstract309)      PDF (7855KB)(536)      
Sixty-eight varieties of Brassica campestris ssp. chinensis were tested and screened for heat tolerance based on phenotype evaluation in field, and varieties with various heat tolerances were measured for their malondialdehyde content, relative leaf membrane permeability and proline content. The results showed the five tolerant varieties and five sensitive varieties selected were increased in malondialdehyde content, relative leaf membrane permeability and proline content under heat stress as time proceeded, but significant differences were found between the two groups of varieties. Therefore, malondialdehyde content, membrane permeability and proline content could be used as physiological indices for identification of heat tolerance in Brassica campestris ssp. chinensis.
Effects of Nitrogen Application Amount on Formation and Yield of Potato Tuber Starch in Ningxia Arid Region
LIU Qiangjuan, KANG Jianhong, WU Jiarui, SUN Jianbo, MA Xueying, WANG Xingqiang, JIAN Tiancai
2021, 35 (5): 1196-1208. DOI: 10.11869/j.issn.100-8551.2021.05.1196
Abstract92)   HTML1)    PDF (1930KB)(1023)      
For exploring the effect mechanism of different nitrogen application amounts impact on potato tuber starch formation and yield in Ningxia arid areas, field trials were carried out in the mountainous areas of southern Ningxia in 2017(normal year) and 2019(wet year) respectively. The potato cultivar Qingshu No. 9 was used as the material and a single factor random Block experiment was designed, with no nitrogen fertilizer (N0) as the control, to study the effects of pure nitrogen application rate of 75 (N1), 150 (N2), 225 kg·hm -2 (N3) on potato starch content, key starch forming enzymes and yield. The results showed that with the increase of nitrogen application amount, the total starch and amylopectin content increased first and then decreased, with N2 being the highest, significantly increasing by 21.23, 26.42 percentage point (2017) and 19.74、24.93 percentage point(2019); while the amylose content increased with the increase of nitrogen application amount, reaching the highest at N3, which was 1.59 percentage point (2017) and 1.78 percentage point (2019) higher than N0. Both adenosine diphosphate glucose pyrophosphorylase (AGPP) and starch branching enzyme (SBE) had the highest N2, which increased by 57.38%, 36.83% (2017) and 67.19%, 29.01% (2019) compared with N0, respectively; while soluble starch synthase (SSS) and bound starch synthase (GBSS) were the highest with N3, which increased by 33.90%, 49.51% (2017) and 46.02%, 51.00% (2019) respectively compared with N0. Correlation and general analysis show that starch content and key enzymes in starch synthesis have a positive correlation, and AGPP, SSS, and GBSS have a greater direct contribution to the process of starch synthesis; Compared with N0, the potato output of N1~N3 increased by 10.72%~21.02% and 8.40%~16.30% in 2017 and 2019, respectively. The yield of N2 is the best; the best nitrogen application rate to achieve the highest yield is 150-184 (2017), 150-196 kg·hm -2 (2019). Therefore, it is suggested that the nitrogen application amount of 150-200 kg·hm -2 for potato in arid areas of Ningxia can effectively increase the activity of key enzymes in starch formation, accelerate starch formation and accumulation, and promote the increase of potato production, thereby increasing the economic benefits of local people.
Transcriptome Analysis and Gene Function Annotation of Early Developmental Broccoli Microspores Based on High-throughput Sequencing Technology
ZHANG Zhenchao,YAO Yuemei,MAO Zhongliang,SUN Guosheng,QIN Wenbin,DAI Zhongliang
2018, 32 (5): 848-855. DOI: 10.11869/j.issn.100-8551.2018.05.0848
Abstract173)   HTML1)    PDF (9287KB)(935)      
In order to investigate the molecular biological mechanisms of broccoli microspore embryogenesis, the Illumina HiSeq TM 2000 platform was utilized to analyze and annotate transcriptomes of the microspores, cultured at 32.5℃for 17 h and 24 h and at 25℃ for 0, 1, 6 d in this research. The results showed that a total of 174324 Unigenes were produced, among which 144 194 were annotated in GO、COG、KEGG、NR、Swissprot and Pfam database. The analysis of gene ontology function enrichment revealed that some important biological processes related to microspore embryogenesis, such as cell part, cell and organelle, binding and catalytic activity, metabolic process, cellular process and single-organism process, etc, got significantly enriched. The frequencies of R、L、K、T and O were much higher than others in COG function classification. The results of KEGG pathways enrichment showed that differentially expressed genes produced from microspores after heat shock tended to cluster into the pathway of protein processing in endoplasmic reticulum, the pathways of plant-pathogen interaction, plant hormone signal transduction and spiceosome, etc. The results laid a good foundation for further study on the physiological and biochemical and molecular mechanisms of broccoli microspore embryogenesis.
Stable Isotope and Mineral Element Characteristics and Construction of the Geographic Identification Protection Model of Pujiang Grape
SHEN Xue, YUAN Yuwei, NIE Jing, LI Chunlin, SHAO Shengzhi, SHE Junyan, WU Yun, ZHANG Yongzhi
2022, 36 (1): 105-113. DOI: 10.11869/j.issn.100-8551.2022.01.0105
Abstract51)   HTML1)    PDF (2705KB)(150)      

In order to explore the feasibility of mineral elements and stable isotopes in the identification of grape origin, representative samples from 4 different producing areas in Pujiang and Zhejiang (Cixi, Wenling), Shanghai and Anhui was collected. Mineral element content and stable isotope ratio of grape samples were determined by inductively coupled plasma-mass spectrometry (ICP-MS) and elemental analyzer-stable isotope ratio mass spectrometry (EA-IRMS), and the origin identification were discussed by chemometrics. The results showed that the δ13C、δ2H and δ18O in Pujiang grape were significantly different from Anhui grape, while not significantly different from other producing areas in Zhejiang and Shanghai. At the same time, there was no significant difference in Al, Na, Mo, V and Sr between other one or two producing areas, whereas the other mineral elements are significantly different in each producing area.The origin discrimination model of Pujiang grape was established by PLS-DA, in which the discrimination accuracy of Pujiang grape reached 100% and the overall discrimination accuracy reached 75%. The results of this study provide reference methods and research ideas for discriminating grape producing areas on a small spatial scale.

GUO Hai+1 ZHAO Ming+2 LI Yun+1 LIANG Ji+1 LI Hui+1 CHEN Xiao yang 1 (1.College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083; 2. Wageningen University, Wageningen, Netherlands)
2004, 18 (05): 368-371+384. DOI: 10.11869/hnxb.2004.05.0368
Abstract956)      PDF (173KB)(1678)      

The effects of changed CO 2 concentration in greenhouse environment or culture container on plant growth, and the techniques of using CO 2 in culture container of tissue culture are introduced.The questions and prospects of CO 2 application in tissue culture are discussed.

Marker Encryption and Candidate Gene Prediction of Sugar Content-Related QTL in Pumpkin
WANG Manman, QU Shuping, HUANG Hexun, XUE Shudan, WU Tingquan, LI Junxing, DAI Zuyun, ZHONG Yujuan
2020, 34 (11): 2407-2415. DOI: 10.11869/j.issn.100-8551.2020.11.2407
Abstract183)   HTML2)    PDF (5884KB)(520)      
In order to understand the inheritance of sugar-related traits in pumpkin fruit, two high generation of inbred lines with significant difference in sugar content and composition, the guangdong local high line (CMO-97) and Thailand line (CMO - E), were used as the parents to build F 2 segregation population in this study. Based on the high-throughput sequencing and parental genome resequencing results, molecular markers linked to the sugar-related trait of the pumpkin were developed. The markers located in the original location mapping region of sucrose and glucose ratio (qs/g19-a) on the genetic map was encrypted. The key genes controlling sweetness of pumpkin were predicted and molecular markers closely linked to fruit sugar content were screened. The results showed that 9 pairs of InDel markers and 6 pairs of KASP markers were polymorphic among the 50 pairs of InDel markers and 12 pairs of KASP markers. The encrypted linkage group 19 contained 6 KASP markers, 9 InDel markers and 112 SNP markers. The qs/g19-a localization region was narrowed from 968.7 to 356.3 kb. Compared with the reference genome information, we found that there were 56 genes in the narrowed localization region, among which there were 4 candidate genes related to sugar content. The results of this study provide reference and molecular marker resources for breeding studies on pumpkin sugar content.
Nitrate Uptake, Transport and Signaling Regulation Pathways
LI Chenyang, KONG Xiangqiang, DONG Hezhong
2020, 34 (5): 982-993. DOI: 10.11869/j.issn.100-8551.2020.05.0982
Abstract233)   HTML2)    PDF (3084KB)(1048)      

Nitrate is the major forms of nitrogen that plants absorb from the soil, and its uptake and utilization is a highly coordinated and complex process. For the sake of survive in various environmental conditions, plants have evolved suitable nitrate uptake and utilization mechanisms to adapt to a wide range of environmental conditions. There are various types of nitrate receptors in plant roots, which can sense different concentrations of external nitrate. The low- or high-affinity nitrate uptake system was activated to absorb nitrate from the environment according to the levels of external nitrate. Once nitrate is taken up into root cells, most of it is transported to shoots for assimilation, and synthesizes macromolecular substances to optimize plant growth. When the nitrate supply is too much for use immediately, the plant can store the excess nitrate into vacuoles and mediate it efflux from vacuoles to the cytosol for assimilation when needed. During plant growth and development, nitrate in the old and mature leaves can be redistributed into developing tissues to promote their growth. Many genes related to nitrate absorption, transportation, storage, assimilation and signaling regulation are activated orderly and work coordinately to absorb and utilize nitrate efficiently. This review summarizes the NRT1 and NRT2 nitrate uptake-related genes and their functions, as well as related transcription factors involved in primary nitrate response and small signal peptides in nitrate signaling transduction and exchange among different tissues. In order to further understand the mechanisms of plant uptake and utilize nitrate, and therefore provides new ideas in increasing nitrogen-use efficiency of crop by breeding and cultivation techniques.

Cloning and Deletion Analysis of NtMTPC4 Promoter From Nicotiana tabacum
LIU Jikai, ZHANG Lin, GAO Yongfeng, WU Chanjuan, TANG Yunlai
2017, 31 (7): 1282-1289. DOI: 10.11869/j.issn.100-8551.2017.07.1282
Abstract159)   HTML0)    PDF (6607KB)(576)      
Tissue-specific promoters are important tools in genetic engineering and practical application. In order to study the expression characteristics of NtMTPC4 promoter from Nicotiana tabacum, the relative expression of NtMTPC4 was determined by quantitative real-time PCR (qRT-PCR). A 2 287 bp length promoter sequence of NtMTPC4 gene was cloned by PCR method. Based on the distribution of the regulatory elements, three truncations of NtMTPC4-P were obtained. The full length NtMTPC4-P and the truncations were then inserted into pBI121K respectively to replace the CaMV35S promoter. All the recombinant plasmids were introduced into Arabidopsis by Agrobacterium-tumefaciens mediated floral dipping method. qRT-PCR analysis indicated that NtMTPC4 gene was highly expressed in flowers. Sequence analysis showed that NtMTPC4-P contained basic cis-elements, such as CAAT-box, TATA-box and tissue specific regulation elements, GTGANTG10 and POLLEN1LELAT52. GUS histochemical assay showed that the expression of the GUS in all of the transgenic plants was detected particularly in pollens. These results provide a new regulatory element for the expression of target gene specifically in pollen by genetic engineering.
Comparative Study on Sugar Metabolism in Keitt Mango Fruit Between Bagging With Yellow and White Bags During Postharvest
WU Lina, ZHU Yuyan, HUAN Chen, XU Qihang, LI Sheng'e, ZHENG Xiaolin
2021, 35 (5): 1121-1128. DOI: 10.11869/j.issn.100-8551.2021.05.1121
Abstract100)   HTML1)    PDF (3533KB)(461)      
In order to explore the effects of different colored bagging on postharvest soft nose and storage characteristics, the incidence of soft nose and sugar metabolism in mango fruit Mangifera indica Keitt pre-harvest bagged with yellow or white bag were investigated during storage. The results showed that the bagging with yellow bag significantly reduced the incidence of soft nose disease in mango fruit during storage for 10 d compared to bagging with white bags. In addition, the bagging with yellow bags significantly decreased the activities of amylase, acid invertase (AI), neutral invertase (NI), sucrose phosphate synthase (SPS) and sucrose synthase (SS) in flesh of the mango fruit, which resulted in slowing the rates of decrease in starch content and accumulation of soluble sugar content, and in turn to be contributed to delaying the softening rate and improving the fruit storability of mango fruit during storage. It was suggested that the bagging treatment affected the sugar metabolism would be associated with the development of soft nose disease in Keitt mango fruit during postharvest. Our present work might provide references for selecting reasonable pre-harvest bagging materials of mango fruit in practice.
Drought Resistance Evaluation Based on Leaf Anatomical Structure of Major Chestnut Cultivars in Beijing-Tianjin-Hebei Region
GUO Yan, ZHANG Shuhang, LI Ying, ZHANG Xinfang, WANG Guangpeng
2021, 35 (8): 1771-1782. DOI: 10.11869/j.issn.100-8551.2021.08.1771
Abstract103)   HTML7)    PDF (6829KB)(198)      
To explore the evaluation method of drought resistance of Chestnut cultivars. In this study, 21 anatomical structure indexes of 36 chestnut cultivars were measured by using multi-function image analysis, frozen sectioning and nail oil seal method combined with the determination of leaf water retention capacity. Leaf anatomical structure indexes were screened by variance analysis and principal component analysis, and comprehensive evaluation on drought resistance of each cultivar was conducted by the subordinate function.The results showed that 18 anatomical structure indexes of the 21 indexes all reached extremely significant differences among the 36 cultivars ( P<0.01), and 6 typical indexes including leaf thickness, thickness of palisade tissue, the total stoma perimeter per unit area, petiole length, thickness of upper epidermis cell and spacing length of veins were selected from the 18 indexes by principal component analysis. The drought resistance level of 36 cltivars were divided into 5 types according to the measurement value of drought resistance ( D)calculated by the membership function method combined with 6 typical indexes and their weights, 4 cultivars were divided into high drought resistance types(HR),the D value ranged from 0.60 to 0.80; 10 cultivars were divided into drought resistance types(R), the D value ranged from 0.50 to 0.59; 13 cltivars were divided into medium drought resistance types(MR), the D value ranged from 0.30 to 0.49; 6 cltivars were divided into low drought resistance types(LR),the D value ranged from 0.20 to 0.29; 3 cltivars were divided into susceptible types(S),the D value ranged from 0 to 0.19. The classification of 36 chestnut cultivars based on the drought resistance measurement were consistent with the evaluation of leaf water retention. This study provided basis for the drought resistance type division and scientific utilization of chestnut cultivars in Beijing-Tianjin-Hebei region.
Association Analysis of Important Agronomic Traits in Pepper ( Capsicum annuum L.) and Mining of Elite Alleles
YUAN Xinjie, FANG Rong, ZHOU Kunhua, LEI Gang, HUANG Yueqin, CHEN Xuejun
2020, 34 (12): 2658-2672. DOI: 10.11869/j.issn.100-8551.2020.12.2658
Abstract178)   HTML0)    PDF (1694KB)(321)      
In order to explore the associated loci and elite alleles for important agronomic traits of pepper, including plant height, first flower node, fruit weight, fruit length, fruit diameter, fruit shape index and pericarp thickness, the genotyping data of 58 SSR markers which distributed on 12 chromosomes in 194 Capsicum annuum core collections were used to detect association with 7 phenotypic traits by using GLM (general linear model, GLM) and MLM(mixed linear model, MLM) methods. The results showed that 20 associated SSR loci were detected by GLM methods, which explain 2.09%~21.91% of the phenotypic variance; 17 associated SSR loci were detected by MLM methods, which explain 2.07%~7.24% of the phenotypic variance; 12 loci were detected by both methods; elite alleles such as TC7268Sa, CAMS-327a, HpmsE132a, ge35-141pmH0135Ca, CAMS-454c and Hpms2-24a were explored based on the phenotypic effect of each associated locus; 28 typical excellent carrier materials, including germplasm B003, B010, B015, B020, B022, B042, B048, B052, B097, B111, B134, B138, B166, B178, B351, B352, C005, C014, V06C0007, V06C0295, V06C1082, V06C1088, V06C1187, V06C1321, V06C1600, V06C1707, V06C1719 and V06C1898 were selected based on these elite alleles. The results in this study provided theoretical guidance and valuable natural resources for the excavation of excellent genes, marker-assisted selection breeding and pyramiding breeding for related agronomic traits in pepper.
The Bacterial Community Diversity of Traditional Fermented Yak Milk in Gannan of Gansu
MA Caixia, LIANG Qi, WANG Xiangzhu, LIU Ying
2022, 36 (1): 154-162. DOI: 10.11869/j.issn.100-8551.2022.01.0154
Abstract46)   HTML0)    PDF (2400KB)(92)      

To investigate the bacterial diversity of the traditional fermented yak milk in the Gannan area of Gansu province, the V3-V4 regions of 16S rRNA from 13 fermented yak milks were sequenced using Illumina MiSeq high-throughput sequencing technology to analyze the bacterial flora diversity of traditional fermented yak milks in Gannan, Gansu Province. The results showed that 720 221 high-quality 16S rRNA gene sequences were generated and four different bacterial phyla and four different bacterial genera were identified. Firmicutes was the dominant phylum, Lactobacillus was the high abundance genus, and yak yogurt from different sources showed different abundance at the phylum level. PICRUSt analysis based on 16S rRNA sequencing data of each sample revealed that the basic predicted function accounted for 11.76% of all genes. This study can build a basis for the protection and utilization of microbial resources in the traditional fermented yak milk in Gannan, Gansu.

Effect of 60Co-γ Irradiation on Capsule of Epidendium secundum
ZHOU Yaqian, YAO Na, WEI Li, LI Lubin, LIU Lei
2017, 31 (9): 1693-1699. DOI: 10.11869/j.issn.100-8551.2017.09.1693
Abstract197)   HTML1)    PDF (1220KB)(884)      
To investigate the effect of the 60Co-γ ray treatment on the Epidendrum secundum capsule, different radiation doses of 60Co-γ ray (0 to 200 Gy) were used in this study, and the effect of radiation on the seeds germination and the seedling growth were investigated. The results showed that 20 Gy radiation dose can promote the germination rate compared with the control group, and the germination time was shortened. Under the radiation dose of 200Gy, the seeds could not germinate. The semi-lethal dose (LD 50) was 78.08 Gy. The biological statistics analysis indicated the radiation dose had larger impact on plant height and leaf length, and had little effect on blade quantity and less influence on the leaf width. These results will lay the theory basis for radiation breeding of Epidendium secundum.
Research Advances in Plant Stress Resistance Regulated by Signal Molecule Hydrogen Sulfide
TIAN Yun, JIANG Jinglong, LI Li, YU Miao, REN Xuming
2017, 31 (11): 2279-2287. DOI: 10.11869/j.issn.100-8551.2017.11.2279
Abstract169)   HTML1)    PDF (4316KB)(843)      
Hydrogen sulfide(H 2S) is a novel type of active endogenous signal molecule following carbon monoxide(CO) and nitric oxide(NO), which plays an important role in plant seed germination, photosynthesis stomatal movement, lateral root formation and delaying plant senescence. Effects of H 2S on plant resistance to biological or abiological stress have been reported in many papers. The objective of this study was to review the molecular mechanism of H 2S synthetic metabolic pathway and the research advances in H 2S alleviating various plant stressses, including heavy metals, high salt, low oxygen, drought, high temperature and low temperature and so on. The molecular mechanism of interaction between H 2S and other signal molecules was also discussed, which is intended to provide a reference for further study of the mechanism of H 2S.
THE EFFECTS OF HIGH-pH AND IRON ON ROOT Fe~(3+) REDUCTASE ACTIVITY OF Rhododendron mucronatum AND Rhododendron simsii
SUN Zhen-yuan~1 XU Wen-zhong~2 ZHAO Liang-jun~3 LIU Shu-lan~3 (1.Research Institute of Forestry,Chinese Academy of Forestry/Key Laboratoryof Forest Cultivation,State Forestry Administration,Beijing,100091;2.Institute of Botany,Chinese Academy of Science,Beijing,100093;3.China Agricultural University,Beijing,100094)
2005, 19 (06): 456-460. DOI: 10.11869/hnxb.2005.06.0456
Abstract1131)      PDF (294KB)(1458)      

The regenerated tissue culture seedlings of Rhododendron mucronatum and R. simsii,which had different alkalinity-tolerance,were used to study the relationship between the root ferric reductase activity and iron and high-pH. The results indicated that:(1) High-pH(provided by NaHCO_3) combined with iron element induced the increase of root ferric reductase activity during the beginning of treatment.The induction of Fe~(3+) on the root ferric reductase activity was very similar as the effect of substrate induction in some metabolic pathway;(2) The root ferric reductase activity of R.simsii increased,however,that of R. mucronatum didn't increase during ten days Fe-deficiency treatment.This results showed that the induction of the root ferric reductase activity by Fe-deficiency is different between different species;(3) There was positive relativity between root ferric reductase activity and alkalinity-resistance of Rhododendron.

Role of Alternative Splicing in Plant Development and Abiotic Stress Responses
FENG Yalan, XIONG Ying, ZHANG Jun, YUAN Jiale, CAI Aishan, MA Chao
2020, 34 (1): 62-70. DOI: 10.11869/j.issn.100-8551.2020.01.0062
Abstract183)   HTML1)    PDF (1726KB)(763)      
Alternative splicing is one of the molecular mechanisms by which plants regulate their gene interaction networks. It regulates plant development and response to abiotic stresses mainly at the post-transcriptional level, greatly increasing the complexity of the transcriptome and proteome. This review summarizes the molecular mechanisms and action pattern of alternative splicing, as well as its role in plant development and abiotic stress responses. Based on current research status, the future research direction of alternative splicing has being prospected, which may provide theoretical reference for the regulation of plant growth and development as well as the breeding of excellent stress-resistant varieties.
Effects of Herbivore Induced Tobacco Resistance on Feeding and Detoxification Enzymes of Spodoptera litura (Lepidoptera: Noctuidae)
YU Yuanchan, YANG Maofa, SHANG Shenghua, LIU Jianfeng, YU Xiaofei, DI Xueyuan
2022, 36 (1): 236-243. DOI: 10.11869/j.issn.100-8551.2022.01.0236
Abstract46)   HTML2)    PDF (1648KB)(70)      

In order to investigate the effects of tobacco with different pest-induced resistance on feeding and detoxification physiology of Spodoptera litura, tobacco male sterility K326 was used in this study to observe the feeding selection of S. litura larvae on tobacco induced by Helicoverpa armigera, S. litura and Myzus persica, mechanical treatments(pricking, needling) and untreated tobacco as positive and negative controls. The detoxifying enzymes activities of the following feeder S. litura containing acetylcholinesterase (AchE), carboxylesterase (CarE), glutathione-S-transferase (GST), multifunctional oxidase (MFO) were detected and analyzed. The results showed that the newly hatched larvae of S. litura preferred to eat the positive contrast leaves induced by mechanical pricks and aphids, while the 3rd instar larvae preferred the positive contrast (punch) leaves relative the S. litura. The AchE activity of S. litura was induced after feeding tobacco leaves, the AchE in aphid processing was exhibited the maximum increase, which was 65.17% higher than that in CK at 48 h. Compared with CK, the H. armigera, S. litura and aphid treatments induced CarE, MFO, and GST at 48, 48 and 4 h increased 14.38%, 72.22% and 47.47% respectively. The activities of MFO and GST, CarE, MFO increased at 12 h and 48 h, 48 h, 4 h, respectively, and there was no significant difference compared with CK (P> 0.05). GST activity of S. litura and CarE activity of M. persicae were significantly inhibited at 48 h, only 55.67% and 81.05% of CK. This indicated that different resistant tobaccos of S. litura have different feeding rates. Feeding resistant tobaccos could activate the AchE of S. litura. Other enzyme under different resistance treatments showed specific changes over time. This study can provide ideas and research basis for integrated control of S. litura in tobacco fields.

Application of CRISPR/Cas9 Gene Editing Technology in Crops
LIN Mengmeng, LI Chunjuan, YAN Caixia, SUN Quanxi, ZHAO Xiaobo, WANG Juan, YUAN Cuiling, SHAN Shihua
2021, 35 (6): 1329-1339. DOI: 10.11869/j.issn.100-8551.2021.06.1329
Abstract135)   HTML8)    PDF (2443KB)(254)      
CRISPR/Cas9 is a genome directed editing technique mediated by small guide RNA. Since 2005, the technology has been developed rapidly and was widely used in biology, medicine and crop genetics and breeding. Compared with meganucleases, zinc finger nucleases (ZFNs) and transcription-like activator effector nucleases (TALENs), CRISPR/Cas9 technology has unsurpassed advantages and has become the most important gene editing technology at present due to its simple operation and high editing efficiency. This paper systematically described the origin, development and the characteristics of CRISPR/Cas9 gene editing, summarized the application of this technology in crop gene editing and other aspects. The purpose of this paper is to provide reference for crop germplasms innovation, gene mining and breeding.
Metabonomics Analysis of Metabolic Difference of Hunan Tea [ Camellia Sinensis (L) O. Kuntze] Germplasm Resources
LIU Zhen, CHENG Yang, ZHAO Yang, YANG Peidi, NING Jing, YANG Yang
2022, 36 (1): 83-93. DOI: 10.11869/j.issn.100-8551.2022.01.0083
Abstract71)   HTML2)    PDF (7945KB)(80)      

Hunan province is located in a transitional zone of tea evolution, where tea germplasms resources are abundant. These germplasm have not only significantly different botanical characteristics, but also varied tea suitability, adaptability, cold tolerance, and etc. However, there were insufficient analyses on the component differences of tea resources in Hunan province. In order to further explore the differences in functional component of Hunan local tea germplasm, and provide the basis for mining and utilizing these tea resources. The metabolites of Chenbu Dongcha (CBDC), Jianghua Kucha (JHKC), Rucheng Baimaocha (RCBM), and Anhua population (AHQT) in Hunan province were detected using UPLC-MS/MS (with the cultivated landrace AHQT as the control group) and identified using mass spectrometry database. A total of 609 metabolites were detected in the 4 populations. Principal component analysis (PCA) showed that the metabolites among the 4 populations were significantly different. The metabolites of AHQT population had the highest similarity to that of CBDC population, and the lowest to that of RCBM populations. With AHQT population as the reference, a total of 302 differential metabolites were detected based on the OPLS-DA model, mainly including amino acids, phenolic acids, flavonoids, tannins, and alkaloids. Among which fifty common differential metabolites were detected in the 3 populations; they were mainly flavonoids and phenolic acids. There were 52, 37, and 66 unique differential metabolites that were detected in CBDC, JHKC, and RCBM, respectively. Except for some reported differential metabolism, we also detected some less concerned differential metabolites in tea research. These differential metabolisms will provide an important reference for tea germplasm excavation and breeding.

Review on Chemical Structures and Biological Activities of Spermidine Derivatives in Plants
ZHAO Shuo, XING Cencan, WANG Yan, SUO Ran, WANG Fengzhong
2018, 32 (1): 123-130. DOI: 10.11869/j.issn.100-8551.2018.01.0123
Abstract155)   HTML0)    PDF (1167KB)(678)      
Spermidine derivatives, which widely existed in the pollen, leaf and fruit in the plants, are a kind of natural compounds which the hydrogen atom of spermidine is replaced by other functional group. The structural identification, purification, especially the biological activities of spermidine derivatives are still worthy to explore. This review summarizes the chemical structures, sources, purification and biological activities of spermidine derivatives reported in the past 30 years, which may possibly build a foundation for future research of spermidine derivatives with special functions.
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