%0 Journal Article %A LOU Qiaoming %A LI Zhe %A LIU Lianliang %A ZHANG Jinjie %A YANG Wenge %A XUE Changhu %T Silylation Derivatization of Monoglyceride and Analysis by Gas Chromatography-Mass Spectrometry %D 2020 %R 10.11869/j.issn.100-8551.2020.11.2519 %J Journal of Nuclear Agricultural Sciences %P 2519-2525 %V 34 %N 11 %X In order to analyze the monoglyceride compositions of Asterias rollestoni gonad, the derivative methods and chromatographic conditions of monoglycerides were optimized, and the fragmentation rules and mass spectrometry characteristics of monoglyceride derivatives were analyzed and monoglyceride compositions in Asterias rollestoni gonad were also detected in this paper. The results indicated that trimethylsilylation had ideal derivative effects on monoglycerides, and the ideal chromatographic separation effects of monoglyceride derivatives were obtained by the weak-polar glass capillary column (HP-5MS, 30 m × 0.25 mm × 0.25 μm). Moreover, the characteristic ions of 1-monoglyceride derivatives were m/z 73, m/z 205, [M-103]+ (base-peak ion) and [M-15]+, while 2-monoglyceride derivatives were m/z 73 (base-peak ion), m/z 129, m/z 218, [M+H-162]+ and [M-15]+. Gas chromatographic retention time of monoglyceride derivatives displayed certain regularitys, and 2-monoglyceride derivatives were eluted earlier than 1-monoglyceride derivatives. Additionally, eight monoglycerides were identified from Asterias rollestoni gonad, which were mainly consisted of 1-monopalmitin (1-C16:0-MG) and 1-monostearin (1-C18:0-MG), while the contents of 2-monoglycerides were relatively low. This research provides theoretical references for derivatization, chromatographic analysis and mass spectrometric identification of monoglycerides. %U https://www.hnxb.org.cn/EN/10.11869/j.issn.100-8551.2020.11.2519