%0 Journal Article %A XU Yuanfang %A LI Wenge %A DENG Gangqiao %A CHEN Shixin %A ZHANG Qiling %A ZHOU Yiji %A ZHANG Yong %A PENG Ling %T Effect of 60Co-γ Ray and Electron Beam Irradiation Sterilization on Antioxidant Activity and Fingerprint of Pueraria Extract %D 2020 %R 10.11869/j.issn.100-8551.2020.08.1713 %J Journal of Nuclear Agricultural Sciences %P 1713-1721 %V 34 %N 8 %X To evaluate the effect of 60Co-γ ray and electron beam irradiation sterilization on the quality of pueraria extract, samples are treated with 60Co-γ ray irradiation(0, 2.8, 5.7, 8.3, and 10.6 kGy) and electron beam irradiation(0, 2.4, 5.1, 7.7, and 10.3 kGy) to investigate the microbe quantity, antioxidant activity and fingerprint. The result showed that both 60Co-γ ray and electron beam irradiation could effectively reduce the total aerobic bacteria count, the total yeast and mold count in pueraria extract. With the increase of absorbed dose, the inhibitory effect was enhancedand. The pueraria extract treated by the two kinds of irradiation methods both reached the microbial limits standard stipulated in the Chinese pharmacopoeia, and the total aerobic bacteria count is 103 CFU·g-1 and the total yeast and mold count is 102 CFU·g-1, when the absorbed dose of 60Co-γ ray and electron beam irradiation were 5.7 kGy and 5.1 kGy, respectively. While the absorbed dose were 8.3 kGy and 7.7 kGy, respectively, the total aerobic bacteria count and the total yeast and mold count were both reduced to below 10 CFU·g-1. In addition, the escherichia coli and salmonella were not detected. Neither of the two irradiation methods showed significant effects on DPPH free radical (DPPH·) scavenging rate, hydroxyl free radical(·OH) scavenging rate and total reducing ability of pueraria extract(P>0.05). The similarity of characteristic peak of pueraria extract irradiated by 60Co-γ ray and electron beam with different doses reaches 1.0, and the RSD of relative retention time was less than 0.5%, which shows that there were no significant difference in the influence of the two irradiation methods on the fingerprint of pueraria extract. In conclusion, 60Co-γ ray and electron beam irradiation both showed inhibitory effect on the microorganisms of pueraria extract, no significant effect on the antioxidant activity and fingerprint of pueraria extract, and could not affect the efficacy and quality stability of pueraria extract. This research provides scientific reference for the rational application of the two irradiation sterilization techniques, especially electron beam irradiation sterilization utilization in the Chinese medicinal materials, such as decoction pieces and proprietary Chinese medicine of Kudzuvine Root. %U https://www.hnxb.org.cn/EN/10.11869/j.issn.100-8551.2020.08.1713