%0 Journal Article %A SHEN Xiaohui %A JIANG Cheng %A LI Rulai %A LI Jiandong %A ZHANG Hua %A ZHENG Haiyan %A GUO Wei %A SUN Li %A FENG Peng %T Relation Between Cold Resistance and Physiology Change of Alfalfa Root After Mix-sowing With Meadow Fescue %D 2016 %R 10.11869/j.issn.100-8551.2016.10.2065 %J Journal of Nuclear Agricultural Sciences %P 2065-2071 %V 30 %N 10 %X In order to explore the relation between cold resistance and root’s physiological indexes change of alfalfa after mix-sowing with meadow fescue, the tested alfalfa varieties included the Longmu806, the Aohan alfalfa and the Caoyuan 3th. The mix-sowing grass for each combination was meadow fescue (Festuca ovina). Single field with only alfalfa and mix-sowing field of alfalfa with the meadow fescue were made, then the over wintering rate of alfalfa was investigated, and four indicator items relative to the cold resistance, namely soluble sugar, soluble protein, free proline and peroxidase (POD) at alfalfa root in each field was determined. The results showed that the average wintering rate with more than 90% mixed sowing effect better than unicast,and the over wintering rate the each field showed significant difference between signal and mix-sowing with the meadow fescue of Caoyuan 3th and Longmu806, the contents of soluble sugar, soluble protein and free praline in the roots of all three alfalfa varieties increased with decreasing of air temperature in late fall, it decreased with air temperature increasing in the next spring of the following year, the contents of soluble sugar reached peak at the end of October, its average content of the soluble sugar was 15.27% and the soluble protein of Caoyuan 3th and Longmu806 reached peak at the end of October, and Caoyuan 3th mixed seeding had the highest value 21.84 mg·g-1,the soluble protein of Aohan signal and mixed seeding reached peak in the middle of November. For the free praline, its content reached peak in the mid-November, among them the Caoyuan 3th with the meadow fescue was the highest with the value of 21.84 mg·g-1. Following the change of air temperature, the POD activity showed up-down-up trend in the whole winter season. Through the Membership function analysis and the over winter rate investigated, the study showed the order of cold-resistance of alfalfa varieties was as follows: Caoyuan3th+meadow fescue> Longmu806+ meadow fescue > Caoyuan3th >Longmu806>Aohan+meadowfescue>Aohan, and finally to increase the cold-resistance of alfalfa, the alfalfa mix-sowing treatment is better than its single sowing treatment.The results provide theoretical basis and technical support for the adaptability of alfalfa in cold area. %U https://www.hnxb.org.cn/EN/10.11869/j.issn.100-8551.2016.10.2065