Journal of Nuclear Agricultural Sciences ›› 2019, Vol. 33 ›› Issue (6): 1122-1130.DOI: 10.11869/j.issn.100-8551.2019.06.1122

• Food Irradiation·Food Science • Previous Articles     Next Articles

Extraction of Fish Oil From the Byproducts of Bigeye Tuna and Purification of EPA Ethyl Ester by Combined Techniques

SONG Gongshuai1, ZHANG Mengna1, MA Yongjun2, ZHOU Xiaomin2, CUI Yiwei1, DAI Zhiyuan1,3, SHEN Qing1,3,*   

  1. 1 Institute of Seafood, Zhejiang Gongshang University, Hangzhou, Zhejiang 310012;
    2 Zhejiang Xingye Group Co. Ltd., Zhoushan, Zhejiang 316101;
    3 The Joint Key Laboratory of Aquatic Products Processing Technology of Zhejiang Province, Hangzhou, Zhejiang 310012
  • Received:2018-09-17 Online:2019-06-10 Published:2019-04-16

大目金枪鱼加工副产物中鱼油提取制备及EPA分离纯化

宋恭帅1, 张蒙娜1, 马永钧2, 周小敏2, 崔益玮1, 戴志远1,3, 沈清1,3,*   

  1. 1 浙江工商大学海洋食品研究院,浙江 杭州 310012;
    2 浙江兴业集团有限公司,浙江 舟山 316101;
    3浙江省水产品加工技术研究联合重点实验室,浙江 杭州 310012
  • 通讯作者: 沈清,男,研究员,主要从事水产品加工与安全研究。E-mail:leonqshen@163.com
  • 作者简介:宋恭帅,男,主要从事水产品加工与安全研究。E-mail:songgongshuai@163.com
  • 基金资助:
    国家重点研发计划资助项目(2018YFC0311204),国家自然科学基金青年科学基金项目(31601542),浙江省重点研发计划项目(2017C03041),浙江省基础公益技术研究计划(LGN18C200001)

Abstract: In this study, the processed by-products of tuna (T. obesus) was used as the major raw material for preparative separation of fish oil with high content of ethyl type EPA tuna oil by a series of technologies, including optimized extraction of enzyme-assisted extraction of crude fish oil, refinement, chemical ethyl esterification, molecular distillation and urea complexation for preliminary purification, rectification, and silver functionalized material based on separation. The optimal extraction parameters of crude fish oil were as follows: pH was 8.5, enzyme temperature was 45℃, and time was 3 h, and the yield of crude fish oil reached 43.69% under the optimal conditions. The total content of EPA and DHA in purified fish oil was over 80.00%, and the content of EPA and DHA reached 46.34% and 34.58% respectively after being treated by multi-stage molecular distillation and urea complexation treatment. In order to obtain pure EPA ethyl ester, the fish oil was further rectified and the content of EPA ethyl ester reached 82.40% with yield 39.00%. After the preparative separation by silver functionalized material chelating, the content of EPA ethyl ester reached 96.30% with yield 32.90%. This study provides references for the high value utilization of fish processed by-products.

Key words: bigeye tuna, byproducts, fish oil, molecular distillation, chelating

摘要: 为获得较高纯度的乙酯型EPA金枪鱼油,以大目金枪鱼加工副产物为原料,通过响应面法优化酶辅助提取粗鱼油的工艺参数,并采用粗鱼油精制、化学催化乙酯化、多级分子蒸馏和尿素包合初步纯化、精馏高纯化,以及合成银离子功能材料螯合制备分离等一系列技术手段进行制备。结果表明,优化的酶解提取法工艺条件为:酶解pH值8.5、酶解温度45℃、酶解时间3 h,此条件下粗鱼油提取率高达43.69%。乙酯化鱼油经多级分子蒸馏和尿素包合处理后,EPA和DHA总量可达80.00%以上,且分别占46.34%和34.58%。对该鱼油进行精馏试验,获得EPA乙酯,其含量达到82.40%,得率为39.00%,在此基础上,进一步使用银硫醇硅胶制备得到96.30%的EPA乙酯,得率为32.90%。本研究结果为鱼类加工副产物的高值化利用提供了一定参考。

关键词: 大目金枪鱼, 副产物, 鱼油, 分子蒸馏, 螯合