Journal of Nuclear Agricultural Sciences ›› 2021, Vol. 35 ›› Issue (8): 1731-1739.DOI: 10.11869/j.issn.100-8551.2021.08.1731

• Induced Mutations for Plant Breeding·Agricultural Biotechnology • Previous Articles     Next Articles

Construction of Ethyl Methane Sulfonate Mutant Library of Jinmai 47 and Preliminary Analysis of Quality Characteristics of High Generation Mutants

ZHANG Ting**, WEN Hongwei**, YUAN Kai, LU Lahu, SHI Xiaofang, ZHANG Mingyi, JI Hutai, YANG Bin*   

  1. Wheat Research Institute, Shanxi Agricultural University, Linfen, Shanxi 041000
  • Received:2020-04-14 Revised:2020-06-11 Online:2021-08-10 Published:2021-06-11

晋麦47 EMS突变体库的构建及高代突变材料品质性状的初步分析

张婷**, 温宏伟**, 袁凯, 逯腊虎, 史晓芳, 张明义, 姬虎太, 杨斌*   

  1. 山西农业大学小麦研究所,山西 临汾 041000
  • 通讯作者: *杨斌,男,副研究员,主要从事小麦抗旱遗传育种与种质资源创制工作。E-mail:sxxmsyb83@126.com
  • 作者简介:张婷,女,助理研究员,主要从事小麦遗传育种研究。E-mail:zhangting841204@163.com;温宏伟,男,主要从事小麦遗传育种研究。E-mail:sxnkywhw@163.com。**同为第一作者。
  • 基金资助:
    山西省农业科学院博士基金(YBSJJ1811),山西省面上青年基金项目(201801D221314),有机旱作山西省重点实验室开放基金(201805D111015-6),山西省重点研发计划项目(201903D211004-2)

Abstract: To speed up creating of drought-resistant wheat resources and promote the genetic improvement of drought-resistant wheat varieties, the seeds of Jinmai 47 were treated with 0.6% EMS solution, a M2 mutant library containing 12272 individual plants was constructed, and the mutation characters and mutation rates were investigated. In addition, the grain hardness index, protein content, wet gluten content, sedimentation value, water absorption, stability time, maximum resistance and extensive area of 50 M7 and M8 mutants selected in the previous study were analyzed. The results showed that: M2 mutant library was rich in phenotypic variation, including stem character, fertility, spike type, disease resistance, leaf character, growth period and awn character, and the total mutation rate was 30.24%. Among which, the mutation rates of fertility, plant height and panicle shape were higher. There were not only morphological mutations in grain shape, grain length, plumpness and grain color, but also variations in grain quality characters of 50 mutants. Among them, the coefficient of variation of stability time, extensive area, maximum resistance and sedimentation value was the highest, the coefficient of variation of hardness index, protein content and wet gluten content was medium, and the coefficient of variation of water absorption was the lowest. Except for water absorption, positive variation occurred in a certain proportion of other characters. Four mutant materials with good quality characters were preliminarily screened, except hardness index and water γ-absorption, the other six quality characters were better than those of the wild type. This research provides valuable genetic materials for the discovery of wheat functional genes and new ideas for the drought resistance breeding program.

Key words: wheat, ethyl methane sulfonate (EMS), mutant, quality character

摘要: 为加快小麦抗旱种质资源创制,推进抗旱品种的遗传改良工作,采用0.6%甲基磺酸乙酯(EMS)溶液处理晋麦47的种子,构建了包含12 272个单株的M2突变体库,对各突变性状和突变率进行调查与分析。此外,对前期研究筛选出的50份M7和M8突变材料籽粒的硬度指数、蛋白质含量、湿面筋含量、沉降值、吸水率、稳定时间、最大拉伸阻力和拉伸面积共8个品质性状进行了分析。结果表明,M2突变体库表型变异丰富,包括茎秆性状、育性、穗型、抗病性、叶性状、生育期和芒性状等32种突变类型,总突变率为30.24%;其中,育性、株高和穗型的突变率较高。50份突变材料的籽粒不仅产生了粒形、粒长、饱满度和粒色方面的形态突变,其品质性状也发生了不同程度的变异,其中稳定时间、拉伸面积、最大拉伸阻力、沉降值的变异系数最高,硬度指数、蛋白质含量和湿面筋含量的变异系数中等,吸水率的变异系数最小;除吸水率外,其余性状均有一定比例的材料产生了正向变异。初步筛选出4份品质性状优良的突变材料,除硬度指数和吸水率外其余6个品质性状表现均优于野生型。本研究结果为小麦功能基因的发掘提供了宝贵的遗传材料,并为抗旱新品种的选育提供了新的研究思路。

关键词: 小麦, 甲基磺酸乙酯(EMS), 突变体, 品质性状