Journal of Nuclear Agricultural Sciences ›› 2021, Vol. 35 ›› Issue (6): 1394-1401.DOI: 10.11869/j.issn.100-8551.2021.06.1394

• Food Irradiation·Food Science • Previous Articles     Next Articles

Study on Extraction Technology of Nicotine and Neophytadiene From Flue-Cured Tobacco Leaves

DU Kang1, ZHANG Wei1, GU Lili1,*, ZHANG Hua1, HUANG Zhihua2, YAO Wen1   

  1. 1Faculty of Chemical Engineering, Kunming University of Science and Technology, Kunming, Yunnan 650500;
    2Yunnan Tobacco Company Yuxi City Company, Yuxi, Yunnan 653100
  • Received:2020-02-24 Online:2021-06-10 Published:2021-04-16

初烤烟叶中烟碱和新植二烯的提取工艺研究

杜康1, 张尉1, 顾丽莉1,*, 张花1, 黄智华2, 姚雯1   

  1. 1昆明理工大学化学工程学院,云南 昆明 650500;
    2云南省烟草公司玉溪市公司,云南 玉溪 653100
  • 通讯作者: *顾丽莉,女,教授,主要从事化学工程研究。E-mail:liligu001@163.com
  • 作者简介:杜康,男,主要从事天然产物的分离与提纯研究。E-mail:1269324817@qq.com
  • 基金资助:
    中国烟草总公司云南省公司科技计划重点项目(2018530000241026)

Abstract: In order to study the ingredients of Yunnan tobacco essential oil and the extraction tecnology of nicotine and neophytadiene, the typical flue-cured tobacco leaves in Yunnan were used as raw materials to extract nicotine and neophytadiene by CO2 supercritical fliud extraction(CO2-SFE), ultrasonic assisted extraction, Soxhlet reflux extraction and solvent extraction. The composition and content in the extracts were analyzed by gas chromatography-mass spectrometry (GC-MS). The extraction rates and the relative contents of nicotine and neophytadiene in the obtained extracts were compared with different extraction methods and solvents, and the suitable extraction methods for nicotine and neophytadiene in Yunnan flue-cured tobacco leaves were determined. The results showed that the yields of tobacco essential oil from high to low by the four processes were soxhlet extraction, solvent extraction, ultrasound-assisted extraction and CO2-SFE with ethanol as extractant. Nicotine, neophytadiene, n-hexadecanoic acid (Z, Z), 9,12,15-octadecatrienoic acid, 1,5,9-trimethyl-12-(1-methyl ethyl) -4,8,13-cyclotetratriene-1,3-diol and vitamin E were the main components in all the extracts obtained under different extraction processes. The total relative contents of nicotine and neophytadiene were as follows: soxhlet extraction(75.58%, 2.85%) > CO2-SFE(35.01%, 26.80%) > ultrasound-assisted extraction(32.28%, 28.24%) > solvent extraction(9.56%, 45.20%). The results of this study provide a theoretical basis for the study on the ingredients in Yunnan smoke oil, as well as nicotine and neophytadiene.

Key words: extraction, tobacco essential oil, nicotine, neophytadiene, gas chromatography-mass spectrometry(GC-MS)

摘要: 为探究云南烟油的成分以及烟碱和新植二烯的提取工艺,本试验以云南典型初烤烟叶为原料,通过超临界CO2萃取、超声辅助萃取、索式回流萃取和溶剂萃取4种方法提取初烤烟叶中烟碱和新植二烯,采用气相色谱-质谱(GC-MS)分析萃取物组成,比较不同萃取方法和萃取溶剂下,烟叶萃取物得率及所得萃取物中烟碱和新植二烯的相对含量。结果表明,当以纯乙醇作为萃取剂时,4种萃取工艺烟油得率从高到低依次为索式回流萃取>超声辅助萃取>溶剂萃取>超临界CO2萃取。不同提取工艺得到的烟油均检测出烟碱、新植二烯、正十六烷酸、(Z,Z,Z)-9,12,15-十八碳三烯酸、1,5,9-三甲基-12-(1-甲基乙基)-4,8,13-环四十三碳三烯-1,3-二醇、维生素E等主要成分,其中烟碱和新植二烯的相对含量依次为索式回流萃取(75.58%、2.85%)>超临界CO2萃取(35.01%、26.80%)>超声辅助萃取(32.28%、28.24%)>溶剂萃取(9.56%、45.20%)。本研究结果为云南烟油中的成分以及烟碱和新植二烯的纯化工艺研究提供了一定的理论依据。

关键词: 萃取, 烟叶精油, 烟碱, 新植二烯, 气相色谱-质谱(GC-MS)