Journal of Nuclear Agricultural Sciences ›› 2021, Vol. 35 ›› Issue (5): 1067-1073.DOI: 10.11869/j.issn.100-8551.2021.05.1067

• Induced Mutations for Plant Breeding·Agricultural Biotechnology • Previous Articles     Next Articles

Screening of Host Factors and Interaction With Coat Protein P10 of Southern Rice Black Streak Dwarf Virus

ZHANG Ruifang1,2, ZHANG Hehong2, WEI Zhongyan2, CHEN Jianping1,2,*, SUN Zongtao2,*   

  1. 1College of Life Sciences, Fujian Agriculture And Forestry University, Fuzhou, Fujian 350002;
    2Institute of Plant Virology, Ningbo University, Ningbo, Zhejiang 315211
  • Received:2020-02-15 Revised:2020-04-01 Online:2021-05-10 Published:2021-03-16

与南方水稻黑条矮缩病毒外壳蛋白P10互作的寄主因子筛选

张瑞芳1,2, 张合红2, 魏中艳2, 陈剑平1,2,*, 孙宗涛2,*   

  1. 1福建农林大学生命科学学院,福建 福州 350002;
    2宁波大学植物病毒学研究所,浙江 宁波 315211
  • 通讯作者: *陈剑平,男,研究员,主要从事植物病毒学方面研究。E-mail:jpchen2001@126.com;孙宗涛,男,研究员,主要从事植物病理方面研究。E-mail:ztaosun@gmail.com。同为通讯作者。
  • 作者简介:张瑞芳,女,主要从事分子生态学方面研究。E-mail:rfzhang2020@163.com
  • 基金资助:
    国家重点研发计划(2016YFD0200804),宁波市科技创新2025重大专项(2019B10004)

Abstract: In order to understand the biological function of P10 protein of southern rice black-streaked dwarf virus (SRBSDV), we screened rice cDNA yeast two-hybrid library by using P10 protein as a bait, and analyzed the results by the methods of bioinformatics techniques. Ten different clones that may interact with P10 was obtained, including late embryogenesis abundant protein Lea14-A, thioredoxin H1, iron-sulfur assembly protein IscA, eukaryotic translation initiation factor 5A. Their interactions were further confirmed through one by one yeast two hybrid method. These results indicated that the growth and biosynthesis of nutrients in rice might be affected after virus infection. These results will provide a better understanding to of the rice virus pathogenicity.

Key words: southern rice black-streaked dwarf virus, coat protein P10, yeast two-hybrid, protein interaction

摘要: 为找到水稻中与南方水稻黑条矮缩病毒(SRBSDV)外壳蛋白P10(CP)相互作用的寄主蛋白,利用P10筛选水稻膜系统酵母双杂cDNA文库,寻找水稻中与P10互作的蛋白并结合生物信息学技术进行分析,回转验证。结果表明,筛选到10个可能与P10互作的水稻寄主蛋白,如胚发育晚期丰富蛋白Lea14 - A、硫氧还原蛋白TRXh1、铁硫结合蛋白IscA以及真核生物起始翻译因子eIF-5A等。这些蛋白的发现说明水稻受到病毒侵染时,生长发育与营养物质的合成均受到影响。本研究结果为后续进一步研究P10蛋白的功能及其致病机制提供了理论依据。

关键词: 南方水稻黑条矮缩病毒, 外壳蛋白P10, 酵母双杂交技术, 蛋白互作