Journal of Nuclear Agricultural Sciences ›› 2021, Vol. 35 ›› Issue (5): 1039-1047.DOI: 10.11869/j.issn.100-8551.2021.05.1039

• Induced Mutations for Plant Breeding·Agricultural Biotechnology • Previous Articles     Next Articles

Isolation and Preliminary Identification of Salinity Response Related ERFs in Wheat

LI Shijiao1, ZHANG Xiaojun2, QIAO Linyi2, JIA Juqing2, CHANG Lifang2, ZHANG Shuwei2, CHANG Zhijian2,*, LI Xin2,*   

  1. 1School of Bioengineering, Shanxi University, Taiyuan, Shanxi 030006;
    2College of Agriculture, Shanxi Agricultural University, Taigu, Shanxi 030800
  • Received:2020-07-13 Revised:2020-08-21 Online:2021-05-10 Published:2021-03-16

小麦盐胁迫响应相关ERF基因的分离和初步验证

李世姣1, 张晓军2, 乔麟轶2, 贾举庆2, 常利芳2, 张树伟2, 畅志坚2,*, 李欣2,*   

  1. 1山西大学生物工程学院, 山西 太原 030006;
    2山西农业大学农学院,山西 太谷 030800
  • 通讯作者: *畅志坚,男,研究员,主要从事小麦染色体工程与分子育种研究。E-mail:wrczj@126.com;李欣,男,副研究员,主要从事小麦抗病基因资源的挖掘与利用研究。E-mail:18635101299@163.com。同为通讯作者。
  • 作者简介:李世姣,女,主要从事生物技术与育种研究。E-mail:2497475168@qq.com
  • 基金资助:
    主要农作物染色体细胞工程育种-北方冬麦区的新品系、新品种选育(2016YFD0102000),山西省重点研发计划项目(201803D421020、201903D211003),山西省农业科学院应用基础研究计划项目(YGJPY1910)

Abstract: As a part of the AP2/ERF superfamily, Ethylene Response Factor (ERF) family plays a key role in plants' response to salt stress. In order to reduce the negative effect of saline soil on wheat yield, AP2/ERF superfamily was isolated from wheat whole genome. And then, 96 ERF family members, a total of 229 homologous sequences in A, B and D genomes, were identified from the AP2/ERF superfamily according to the clustering results and structural characteristics. By phylogenetic analysis and database-based transcriptomic analysis, 13 TaERF members were selected, which displayed high similarity with the cloned salt-tolerant ERF genes or genes induced by NaCl. Subsequently, salt-tolerant material CH7034 and salt-sensitive variety SY95-71 were used to verify the expression levels of the 13 TaERFs after treatment with 250 mmol·L-1 NaCl. The results showed that TaERF27, TaERF35, TaERF55 and TaERF64 were significantly up-regulated under NaCl stress in CH7034, while there was no significant change in SY95-71, indicating that the four genes may be salt stress response genes. Analysis results of tissue specific expression and promoter regulation element showed that TaERF27, TaERF35, TaERF55 and TaERF64 expressed highly in seedling root and leaf of CH7034, and their promoter (-2 000 bp) sequences all contain multiple elements responding hormones like abscisic acid, salicylic acid and jasmonic acid, which demonstrated that these four TaERF members might be involved in various signal transduction pathways related to abiotic stress in plants. The results of this study not only helps to understand the response mechanism to abiotic stress in plant, but also provides reference genes for the improvement of salt tolerance of wheat varieties.

Key words: wheat, salt stress, ethylene response factor (ERF)

摘要: 隶属于AP2/ERF超家族的乙烯响应因子(ERF)是植物抵御盐胁迫过程中的一类重要基因,为了减轻盐渍土地对小麦产量的负面影响,本研究从小麦全基因组中分离了AP2/ERF超家族,根据聚类结果和结构特征从中鉴定出96个ERF家族成员,在A、B、D基因组中共有229个拷贝序列;通过聚类分析和转录组数据分析筛选出13个与已克隆耐盐ERF基因相似性高或受NaCl诱导的TaERF成员,随后利用小麦抗感材料验证13个TaERF成员在受250 mmol·L-1 NaCl处理后的表达水平变化情况,结果显示,TaERF27、TaERF35、TaERF55和TaERF64在耐盐材料CH7034中受NaCl胁迫后显著上调,而在盐敏感品种SY95-71中无明显变化,推测其可能为盐胁迫响应基因;组织特异表达和启动子调控元件分析结果显示这4个基因在CH7034苗期根和叶中均具有较高的表达水平,并且每个基因起始密码子前 2 000 bp区域内包含脱落酸、水杨酸和茉莉酸等多种植物激素响应元件,推测它们可能参与植物多种非生物胁迫信号转导通路。本研究结果有助于理解植株应对非生物胁迫的分子机制,并为小麦品种耐盐性改良提供了参考基因。

关键词: 小麦, 盐胁迫, 乙烯响应因子(ERF)