Journal of Nuclear Agricultural Sciences ›› 2019, Vol. 33 ›› Issue (7): 1349-1355.DOI: 10.11869/j.issn.100-8551.2019.07.1349

• Food Irradiation·Food Science • Previous Articles     Next Articles

Effects of 60Co-γ Irradiation on the Conformation and Antigenicity of Peanut Allergen Ara h 2

LUO Chunping1, *, HU Chunqiu2   

  1. 1 Taizhou Vocational College of Science & Technology, Taizhou, Zhejiang 318020;;
    2 Department of Nutrition and Food Hygiene, School of Public Health, Anhui Medical University, Hefei, Anhui 230032
  • Received:2018-06-26 Revised:2018-10-04 Online:2019-07-10 Published:2019-05-09

60Co-γ辐照对花生过敏原Ara h 2蛋白构象及致敏活性的影响

罗春萍1, *, 胡纯秋2   

  1. 1 台州科技职业学院,浙江 台州 318020;
    2 安徽医科大学公共卫生学院营养与食品卫生学系,安徽 合肥 230032
  • 通讯作者: *同第一作者。
  • 作者简介:罗春萍,女,讲师,主要从事花生过敏研究。E-mail:went_fly@163.com
  • 基金资助:
    台州市科技计划项目(1701ny13),安徽医科大学博士基金(XJ201015)

Abstract: In order to investigate the effect of irradiation on the changes of structural and antigenic properties of Ara h 2, the separated peanut allergen Ara h 2 was irradiated by 60Co-γ rays at different doses. The structures were characterized by ultraviolet absorption spectrum, circular dichroism (CD), and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), respectively. The antigenicity of the irradiated Ara h 2 was evaluated by Western blotting and indirect ELISA. Results showed that the conformation of Ara h 2 was significantly changed after 60Co-γ irradiation treatment, which caused denaturation and aggregation of Ara h 2. The IgG binding capacity of Ara h 2 was reduced with the increasing irradiation doses, which correlated well with the rise of UV absorption intensity and a loss of α-helix content. Moreover, the structure and antigenicity of Ara h 2 were almost completely destroyed when the irradiation dose was up to 10 kGy. It indicated that 60Co-γ irradiation can effectively reduce the allergenicity of Ara h 2, which may provide a new method on peanut desensitization technology.

Key words: 60Co-γ, irradiation;, peanut allergy, Ara h 2, conformation, antigenicity

摘要: 为探讨辐照处理对花生Ara h 2蛋白结构与致敏活性的影响,采用不同剂量60Co-γ辐照处理分离纯化所得到的花生过敏原Ara h 2蛋白,结合紫外扫描光谱、圆二色谱(CD)和聚丙烯酰胺凝胶电泳(SDS-PAGE)评估辐照处理后Ara h 2蛋白的结构变化,并用免疫印迹法和间接酶联免疫吸附法检测辐照处理后Ara h 2的抗原性变化。结果表明,60Co-γ辐照处理可以显著改变花生Ara h 2蛋白的构象,使其降解、发生交联。随着辐照剂量的增大,Ara h 2蛋白与抗体的结合能力呈逐渐下降趋势,且与蛋白紫外吸光度的增强和α-螺旋含量的降低呈现良好的相关性。当辐照剂量为10 kGy时,可基本破坏 Ara h 2 蛋白的结构和免疫活性。60Co-γ辐照处理可以有效降低花生过敏原 Ara h 2 蛋白的致敏性,这为花生脱敏技术的研究提供了新思路。

关键词: 60Co-γ, 辐照, 花生过敏, Ara h 2, 构象, 抗原性