Journal of Nuclear Agricultural Sciences ›› 2019, Vol. 33 ›› Issue (4): 633-639.DOI: 10.11869/j.issn.100-8551.2019.04.0633

• Induced Mutations for Plant Breeding·Agricultural Biotechnology •     Next Articles

The Study of 60Co-γ Ray Irradiation Effects on Flower Color of Iris hollandica

LIN Bing, ZHONG Huaiqin, HUANG Minling*, FAN Ronghui, LUO Yuanhua   

  1. Fujian Engineering Research Center for Characteristic Floriculture/Flowers Research Center/Institute of Crop Sciences, Fujian Academy of Agricultural Science, Fuzhou, Fujian 350013
  • Received:2018-07-25 Revised:2018-11-20 Online:2019-04-10 Published:2019-01-29

60Co-γ射线辐射对荷兰鸢尾花色诱变效应的研究

林兵, 钟淮钦, 黄敏玲*, 樊荣辉, 罗远华   

  1. 福建省农业科学院作物研究所/花卉研究中心/福建省特色花卉工程技术研究中心,福建 福州 350013
  • 通讯作者: *黄敏玲,女,研究员,主要从事花卉新品种选育与生物技术研究。E-mail: huangml618@163.com
  • 作者简介:林兵,男,助理研究员,主要从事观赏植物引种与栽培研究。E-mail: lb87572540@163.com
  • 基金资助:
    福建省属公益类科研院所专项(2018R1026-6),福建省农业科学院科技创新团队建设项目(STIT2017-2-9)

Abstract: To investigate the mutagenic effects of Iris hollandica in different radiation doses of 60Co-γ rays, the seedballs of zhanchi with dark blue flower were used as test materials and were treated at 0(CK) 3, 5, 7 and 10 Gy doses, respectively, then the growth level and color variation of irradiated progeny were investigated. The results showed that the irradiation had an inhibitory effect on the growth of VM1. However, after two generations of cultivation, the growth index of the VM3 had recovered and variation characteristics were stable. The color mutation effects and color mutation direction were closely releted to the dose of radiation. The receptor appeared white and purple color variations at the 7 Gy treatment, and one-way transformation to white color at low dose of treatment (3 Gy and SGy), while more biased towards purple variation through the semi-lethal dose of 10 Gy treatment. After several generations of breeding, three different lines of variations were selected at VM6 generation, which demonstrats that 60Co-γ ray radiation is a new effective method for new varieties breeding of Iris hollandica. The results of this study laid a theoretical foundation for the breeding of 60Co-γ ray radiation mutation and the selection of new varieties with excellent ornamental traits.

Key words: Iris hollandica, irradiation mutagenesis, color effect, variation selection

摘要: 为探究不同60Co-γ射线辐射剂量对荷兰鸢尾的诱变效应,以花深蓝色的荷兰鸢尾品种展翅种球为试验材料,分别进行0(CK)、3、5、7、10 Gy 5个剂量的辐射处理,并调查辐射后代的生长发育状况以及花色变异表现。结果表明,辐射处理对VM1的生长具有抑制作用,但经过2代的栽培后VM3生长指标恢复,变异特性稳定;受体的花色诱变效应和花色变化产生的方向与辐射剂量密切相关,7 Gy处理时,出现白色和紫色2种颜色;低剂量(3 Gy和5 Gy)处理下的花色更倾向于白色转变,而高剂量(10 Gy)处理下更偏向于紫色方向变化。经多代选育,在VM6中筛选到深蓝紫色、紫罗兰色和白色3个不同花色的变异新株系,表明60Co-γ辐射诱变可以作为荷兰鸢尾新品种培育的有效途径。本研究结果为荷兰鸢尾种球60Co-γ射线辐射诱变育种和选育观赏性状优良的新品种奠定了一定的理论基础。

关键词: 荷兰鸢尾, 辐射诱变, 颜色效应, 变异选择