Journal of Nuclear Agricultural Sciences ›› 2016, Vol. 30 ›› Issue (10): 2065-2071.DOI: 10.11869/j.issn.100-8551.2016.10.2065

• Isotope Tracer Technique·Ecology & Environment·Physiology • Previous Articles     Next Articles

Relation Between Cold Resistance and Physiology Change of Alfalfa Root After Mix-sowing With Meadow Fescue

SHEN Xiaohui1, 2, JIANG Cheng3, LI Rulai1, LI Jiandong2, ZHANG Hua1, ZHENG Haiyan1, GUO Wei1, SUN Li1, FENG Peng1   

  1. 1Jiamusi Branch of Heilongjiang Academy of Agricultural Sciences, Jiamusi, Heilongjiang 154007;
    2 Agronomy College of Shenyang Agricultural University, Shenyang,Liaoning 110866;
    3 College of Life Science, Jiamusi University, Jiamusi, Heilongjiang 154007
  • Received:2015-07-06 Revised:2015-11-10 Online:2016-10-10 Published:2016-12-29

苜蓿与草地羊茅单混播根系生理变化与抗寒性的关系

申晓慧1, 2, 姜成3, 李如来1, 李建东2, 张华1, 郑海燕1, 郭伟1, 孙力1, 冯鹏1   

  1. 1黑龙江省农业科学院佳木斯分院,黑龙江 佳木斯 154007;
    2沈阳农业大学农学院,辽宁 沈阳 110866;
    3.佳木斯大学生命科学学院, 黑龙江 佳木斯 154007
  • 通讯作者: 冯鹏,男,副研究员,主要从事牧草育种及栽培研究。E-mail:fengras@163.com
  • 作者简介:申晓慧,女,助理研究员,主要从事牧草抗寒性研究。E-mail:xiaohuishen@126.com
  • 基金资助:
    黑龙江省政府博士后资助项目 (LBH-Z12239),中国博士后科学基金(2013M541423)

Abstract: In order to explore the relation between cold resistance and root’s physiological indexes change of alfalfa after mix-sowing with meadow fescue, the tested alfalfa varieties included the Longmu806, the Aohan alfalfa and the Caoyuan 3th. The mix-sowing grass for each combination was meadow fescue (Festuca ovina). Single field with only alfalfa and mix-sowing field of alfalfa with the meadow fescue were made, then the over wintering rate of alfalfa was investigated, and four indicator items relative to the cold resistance, namely soluble sugar, soluble protein, free proline and peroxidase (POD) at alfalfa root in each field was determined. The results showed that the average wintering rate with more than 90% mixed sowing effect better than unicast,and the over wintering rate the each field showed significant difference between signal and mix-sowing with the meadow fescue of Caoyuan 3th and Longmu806, the contents of soluble sugar, soluble protein and free praline in the roots of all three alfalfa varieties increased with decreasing of air temperature in late fall, it decreased with air temperature increasing in the next spring of the following year, the contents of soluble sugar reached peak at the end of October, its average content of the soluble sugar was 15.27% and the soluble protein of Caoyuan 3th and Longmu806 reached peak at the end of October, and Caoyuan 3th mixed seeding had the highest value 21.84 mg·g-1,the soluble protein of Aohan signal and mixed seeding reached peak in the middle of November. For the free praline, its content reached peak in the mid-November, among them the Caoyuan 3th with the meadow fescue was the highest with the value of 21.84 mg·g-1. Following the change of air temperature, the POD activity showed up-down-up trend in the whole winter season. Through the Membership function analysis and the over winter rate investigated, the study showed the order of cold-resistance of alfalfa varieties was as follows: Caoyuan3th+meadow fescue> Longmu806+ meadow fescue > Caoyuan3th >Longmu806>Aohan+meadowfescue>Aohan, and finally to increase the cold-resistance of alfalfa, the alfalfa mix-sowing treatment is better than its single sowing treatment.The results provide theoretical basis and technical support for the adaptability of alfalfa in cold area.

Key words: alfalfa, meadow fescue, mixed sowing, wintering rate, physiology index

摘要: 为了探究紫花苜蓿与禾草单、混播越冬期根系生理变化与抗寒性的关系,以龙牧806、敖汉苜蓿、草原3号3个品种为试材,以草地羊茅为混播禾草,在我国黑龙江省东部地区,分别设置苜蓿单播区与苜蓿-草地羊茅混播区,调查苜蓿越冬率,测定苜蓿根系在秋末至翌年春可溶性糖、可溶性蛋白质、游离脯氨酸含量及过氧化物酶(POD)活性等4项指标的动态变化,并运用隶属函数法进行抗寒性综合评判。结果表明,品种不同,苜蓿单混播越冬率表现不一;苜蓿与禾草混播效果好于单播,混播越冬率达到90%以上;草原3号与龙牧806单、混播越冬率差异显著,不同品种之间单播越冬率差异不显著。各品种单播区与混播区苜蓿根系可溶性糖、可溶性蛋白及游离脯氨酸含量均随气温下降而增加,翌年春,随气温回升而降低,其中可溶性糖含量在10月末达到最大值,平均含量为15.27%;草原3号和龙牧806单混播可溶性蛋白在10月末达到最大值,敖汉苜蓿单混播则是在11月中旬达到最高值;游离脯氨酸则在11月中旬达到最高值,且草原3号混播组合值最高,为21.84 mg·g-1。POD活性随温度变化呈现先升后降再上升的变化趋势。供试组合抗寒性大小依次为草原3号+草地羊茅>龙牧806+草地羊茅>草原3号 >龙牧806>敖汉+草地羊茅>敖汉苜蓿。本研究结果为提高紫花苜蓿在高寒地区的适应性提供了理论依据和技术支撑。

关键词: 紫花苜蓿, 草地羊茅, 混播, 越冬率, 生理指标