J4 ›› 2013, Vol. 27 ›› Issue (11): 1777-1782.DOI: 10.11869/hnxb.2013.11.1777

• 论文 • Previous Articles     Next Articles

Effects of Exogenous Nitric Oxide on Germination of Alfalfa Seeds under Water Stress

CAI Zhuo-shan1,2, SHI Shang-li1,2, Xie Seng-lin1, Cai Guo-shan1   

  1. 1. College of Grassland Science, Gansu Agricultural University, Lanzhou, Gansu 730070;
    2. Key Laboratory of Grassland Ecosystem of Ministry of Education/Sino-U.S. Centers for Grazing land Ecosystem Sustainability, Lanzhou, Gansu 730070
  • Received:2013-01-08 Revised:2013-05-03 Online:2013-11-20 Published:2013-11-20

外源NO对水分胁迫下苜蓿种子萌发的影响

蔡卓山1,2, 师尚礼1,2, 谢森林1, 蔡国山1   

  1. 1. 甘肃农业大学草业学院, 兰州 甘肃 730072;
    2. 草业生态系统教育部重点实验室/中-美草地畜牧业可持续发展研究中心, 兰州 甘肃 730070
  • 通讯作者: 师尚礼,男,教授,主要从事牧草种质资源研究。E-mail: shishl@gsau.edu.cn
  • 基金资助:

    牧草种质资源保护利用(NB2130135),国家牧草产业技术体系专项(CARS-35)

Abstract:

The seeds of two alfalfa varieties (Medicago sativa L. cv. Emperor and Medicago sativa L. cv. Debay) pretreated with NO-donor SNP were used to study the physiological effects of exogenous nitric oxide on germination of alfalfa seeds under water-stress. The results showed that germination potential, germination rate, radicle length, embryonic bud length and seeding dry weight of the 2 alfalfa varieties decreased with increasing water stress, while increased with applying exogenous NO. It could be concluded that exogenous NO mitigated inhibition of water stress on germination potential, germination rate, radicle length, embryonic bud length and seeding dry weight of alfalfa, and activated the physiological processes of the seeds germinating.

Key words: Alfalfa, Exogenous nitric oxide, Water stress, Seeds germination

摘要:

以苜蓿王和德宝2 个苜蓿品种为材料,用聚乙二醇(PEG-6000)为渗透调节剂模拟水分胁迫,用一氧化氮(NO)供体硝普钠处理苜蓿种子,研究NO 对水分胁迫下苜蓿种子萌发的生理效应。结果表明,水分胁迫显著抑制了2 个苜蓿品种的发芽势、发芽率、胚根长和胚芽长及幼苗干重(P<0.05),且随着胁迫强度的增加,各萌发指标相应降低,外源NO能够提高水分胁迫下苜蓿种子的发芽势、发芽率、胚芽长及幼苗干重(P<0.05)。因此,水分胁迫抑制了苜蓿种子的萌发,而添加外源NO能够显著降低水分亏缺给苜蓿种子萌发带来的胁迫作用,活化种子萌发生理过程。

关键词: 苜蓿, 水分胁迫, 外源NO, 种子萌发